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anti-FASLG antibody :: Mouse anti-Human Fas Ligand (CD178) Monoclonal Antibody

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Catalog # MBS670167
Unit / Price
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  0.1 mg  /  $230 +1 FREE 8GB USB
anti-FASLG antibody
Product Name

Fas Ligand (CD178) (FASLG), Monoclonal Antibody

Full Product Name

Mouse Anti-Human Fas Ligand-UNLB

Product Synonym Names
Mouse Anti-Human Fas Ligand - Purified, Unlabeled Antibody; Mouse anti-Human Fas Ligand (CD178)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (172628185..172636013). Location: 1q23
OMIM
134638
3D Structure
ModBase 3D Structure for P48023
Clonality
Monoclonal
Isotype
Mouse IgG2b
Clone Number
SB93a1
Host
Mouse
Species Reactivity
Human
Specificity
Reacts with native and recombinant human Fas Ligand1
Fas Ligand (CD178) is a 40 kD type II transmembrane protein that is a member of the tumor necrosis factor superfamily. This well characterized potent apoptotic factor is utilized by cytotoxic T cells and natural killer cells to selectively kill virus infected and tumorigenic cells2,3. Moreover, studies indicate that Fas Ligand is an important regulator in immune homeostasis where it has been shown to down-regulate immune responses during activation-induced cell death4. Fas Ligand initiates apoptosis by binding to its receptor (CD95) on target cells which facilitates recruitment of numerous signaling proteins to form a death inducing signaling complex. This signaling complex is then believed to propagate the apoptotic signal through the recruitment and activation of Caspase- 85.
The SB93a monoclonal antibody detects a band at ~40 kD, corresponding to the transmembrane (insoluble) form of Fas Ligand.
Form/Format
Purified (UNLB) Antibody
Immunogen
Recombinant human Fas Ligand
Characterization
To ensure acceptable performance, each batch of product is tested by Flow Cytometry and/or Western blotting to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.1mg purified immunoglobulin in 0.2mL of 100 mM borate buffered saline, pH 8.0; store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.1mg in 0.2mL of PBS/NaN3. Store at 2- 8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
Other Notes
Small volumes of anti-FASLG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-FASLG antibody
Flow Cytometry; Immunoprecipitation; Western Blot
Application Notes for anti-FASLG antibody
Flow Cytometry Suggested Dilution: <= 1 uL/10^6 cells
Western Blot Suggested Dilution: 0.5-2 ug
Immunoprecipitation Suggested Dilution: 5-20 ug
NCBI/Uniprot data below describe general gene information for FASLG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
31,485 Da[Similar Products]
NCBI Official Full Name
Fas ligand (TNF superfamily, member 6)
NCBI Official Synonym Full Names
Fas ligand (TNF superfamily, member 6)
NCBI Official Symbol
FASLG  [Similar Products]
NCBI Official Synonym Symbols
FASL; CD178; CD95L; CD95-L; TNFSF6; APT1LG1
  [Similar Products]
NCBI Protein Information
tumor necrosis factor ligand superfamily member 6; APTL; CD95 ligand; OTTHUMP00000032708; fas antigen ligand; apoptosis antigen ligand; apoptosis (APO-1) antigen ligand 1; tumor necrosis factor (ligand) superfamily, member 6
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 6
UniProt Synonym Protein Names
Apoptosis antigen ligand; APTL; CD95 ligand; CD95-L; Fas antigen ligand
UniProt Gene Name
FASLG  [Similar Products]
UniProt Synonym Gene Names
APT1LG1; CD95L; FASL; TNFSF6  [Similar Products]
UniProt Entry Name
TNFL6_HUMAN
NCBI Summary for FASLG
The protein encoded by this gene is the ligand for FAS. Both are transmembrane proteins. Interaction of FAS with this ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). [provided by RefSeq]
UniProt Comments for FASLG
Function: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.

Subunit structure: Homotrimer

Probable. Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Ref.12

Subcellular location: Cell membrane; Single-pass type II membrane protein. Secreted. Note: May be released into the extracellular fluid, probably by cleavage form the cell surface.

Post-translational modification: N-glycosylated.The soluble form derives from the membrane form by proteolytic processing.

Involvement in disease: Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [

MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. Ref.9

Sequence similarities: Belongs to the tumor necrosis factor family.
Product References and Citations for anti-FASLG antibody
1) Trambas, C.M. and Griffiths, G.M. (2003) Nat. Immunol. 4:399-403.
3) Voss, M. et al. (2008). Cell. Commun. Signal. 6:11.
4) Krammer, P.H. (2000). Nature. 407:789-95..
5) Peter, M.E. and Krammer, P.H. (2002). Cell. Death Diff. 10:26-35.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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