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anti-CSF3 antibody :: Rat anti-Human G-CSF Monoclonal Antibody

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Catalog # MBS670060
Unit / Price
  0.5 mg  /  $340 +1 FREE 8GB USB
anti-CSF3 antibody
Product Name

G-CSF (CSF3), Monoclonal Antibody

Also Known As

Rat Anti-Human G-CSF-BIOT

Product Synonym Names
Rat Anti-Human G-CSF - Biotin; Rat Anti-Human G-CSF
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
138
Chromosome Location
Chromosome: 17; NC_000017.10 (38171614..38174066). Location: 17q11.2-q12
OMIM
138970
3D Structure
ModBase 3D Structure for P09919
Clonality
Monoclonal
Isotype
Rat IgG2a kappa
Clone Number
BVD11-37G10
Host
Rat
Species Reactivity
Human
Specificity
Human Granulocyte-Colony Stimulating Factor (G-CSF)1,2 (This is a neutralizing antibody.)
Form/Format
Biotin (BIOT) Conjugate
Immunogen
E. coli-expressed recombinant human G-CSF
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
Other Notes
Small volumes of anti-CSF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CSF3 antibody
ELISA; Western Blot
Application Notes for anti-CSF3 antibody
Research Application(s): ELISA Detection: BVD11-37G10 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human G-CSF protein levels.1,2 Biotinylated BVD11-37G10 antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human G-CSF as the standard.
Western Blotting: The purified BVD11-37G10 antibody is also useful for Western blotting applications. A concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant human G-CSF under reducing conditions.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
NCBI/Uniprot data below describe general gene information for CSF3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
22,293 Da[Similar Products]
NCBI Official Full Name
granulocyte colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 3 (granulocyte)
NCBI Official Symbol
NCBI Official Synonym Symbols
GCSF; CSF3OS; C17orf33; MGC45931
  [Similar Products]
NCBI Protein Information
granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin; OTTHUMP00000164325; OTTHUMP00000164326; OTTHUMP00000164327; OTTHUMP00000164328
UniProt Protein Name
Granulocyte colony-stimulating factor
UniProt Synonym Protein Names
Pluripoietin
UniProt Gene Name
UniProt Synonym Gene Names
C17orf33; GCSF  [Similar Products]
UniProt Entry Name
CSF3_HUMAN
NCBI Summary for CSF3
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq]
UniProt Comments for CSF3
Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Post-translational modification: O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

Pharmaceutical use: Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). Used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood).

Sequence similarities: Belongs to the IL-6 superfamily.

Caution: Ref.5 misquotes the gene name as "CSF1".
Product References and Citations for anti-CSF3 antibody
1. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
2. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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