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anti-GPC3 antibody :: Mouse Glypican-3 (GPC3) Monoclonal Antibody

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Catalog # MBS438725
Unit / Price
  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
Product Name

Glypican-3 (GPC3), Monoclonal Antibody

Popular Item
Also Known As

Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Mouse Monoclonal Antibody

Product Synonym Names
DGSX; Glypican proteoglycan 3; GPC3; GTR2-2; Heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI-5; SDYS; Secreted glypican-3; SGBS1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
603
OMIM
300037
3D Structure
ModBase 3D Structure for P51654
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
1G12
Host
Mouse
Species Reactivity
Human.
Others not known
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Cellular Localization
Cytoplasmic
Immunogen
A recombinant fragment containing amino acids 511-580 of human glypican-3
Hu-Chromosome Location
Xq26.2
Positive Control
293T cells or Hepatocellular carcinoma
Preparation and Storage
Antibody without azide store at -20°C to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-GPC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GPC3 antibody
Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC). Hepatoblastoma, melanoma, testicular germ cell tumor, and WilmÂ’s tumor . In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in nomarl live, benign liver and the serum of healthy . GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver o liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as WilmÂ’s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancer: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
Applications Tested/Suitable for anti-GPC3 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-GPC3 antibody
Western Blot (1-2 ug/ml)
Flow Cytometry ( 0.5-1 ug million cells )
Immunofluorescence ( 0.5-1 ug/ml)
Immunohistology (Formalin-fixed)(0.5-1 ug/ml for 30 ming at RT)

(Staining of formalin-fixed tissues requires boiling tissues sections in 10mM Citrate buffer,pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes )

Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-GPC3 antibody
Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Glypican-3 Monoclonal Antibody (1G12)
anti-GPC3 antibody Immunohistochemistry (IHC) (IHC) image
Testing Data (TD) of anti-GPC3 antibody
anti-GPC3 antibody Testing Data (TD) image
Testing Data (TD) of anti-GPC3 antibody
anti-GPC3 antibody Testing Data (TD) image
NCBI/Uniprot data below describe general gene information for GPC3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
67 kDa
NCBI Official Full Name
glypican-3 isoform 1
NCBI Official Synonym Full Names
glypican 3
NCBI Official Symbol
NCBI Official Synonym Symbols
SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
  [Similar Products]
NCBI Protein Information
glypican-3
UniProt Protein Name
Glypican-3
UniProt Synonym Protein Names
GTR2-2; Intestinal protein OCI-5; MXR7
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
GPC3_HUMAN
NCBI Summary for GPC3
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
UniProt Comments for GPC3
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
Product References and Citations for anti-GPC3 antibody
Yan, B., et al. 2011. Expression and clinicopathologic significance of glypican 3 in hepatocellular carcinoma. Ann. Diagn. Pathol. 15: 162-169

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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