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anti-gp100 antibody :: Mouse gp100/Melanosome/PMEL17/SILV Monoclonal Antibody

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Catalog # MBS4380481
Unit / Price
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  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $340 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $340 +1 FREE 8GB USB
Product Name

gp100/Melanosome/PMEL17/SILV, Monoclonal Antibody

Popular Item
Full Product Name

gp100/Melanosome/PMEL17/SILV (Melanoma Marker)

Product Synonym Names
95kDa melanocyte-specific secreted glycoprotein; M-beta; Melanocyte lineage specific antigen GP100; Melanocyte protein Pmel 17; Melanoma associated ME20 antigen; Melanosomal matrix protein17; Premelanosome protein (PMEL17); Secreted melanoma-associated ME20 antigen; SILV; Silver homolog
Product Synonym Gene Name
PMEL17; SILV[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P40967
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
PMEL/2037
Host
Mouse
Species Reactivity
Human. Others not tested.
Specificity
Cytotoxic T lymphocytes (CTL s) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.
Form/Format
200ug/ml of Ab purified from bioreactor concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Immunogen
Recombinant human SILV protein fragment (around aa 376-502)
Cellular Localization
Cytoplasmic
Hu-chromosome Location
12q13.2
Positive Control
SK-MEL-28 cells or Melanoma
Other Notes
Small volumes of anti-gp100 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-gp100 antibody
ELISA (EIA), Immunohistochemistry (IHC) Formalin
Application Notes for anti-gp100 antibody
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-gp100 antibody
Formalin-fixed, paraffin-embedded human Melanoma stained with gp100 Mouse Monoclonal Antibody (PMEL/2037).
anti-gp100 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-gp100 antibody
Formalin-fixed, paraffin-embedded human Skin stained with gp100 Mouse Monoclonal Antibody (PMEL/2037).
anti-gp100 antibody Immunohistochemistry (IHC) (IHC) image
SDS-PAGE Analysis Purified gp100 Mouse Monoclonal Antibody (PMEL/2037).Confirmation of Purity and Integrity of Antibody.
anti-gp100 antibody SDS-Page image
Testing Data of anti-gp100 antibody
Analysis of Protein Array containing more than 19, 000 full-length human proteins using PMEL/gp100 Mouse Monoclonal Antibody (PMEL/2037).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
anti-gp100 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for gp100. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
90-100kDa
NCBI Official Full Name
melanocyte protein PMEL isoform 2
NCBI Official Synonym Full Names
premelanosome protein
NCBI Official Symbol
NCBI Official Synonym Symbols
P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
  [Similar Products]
NCBI Protein Information
melanocyte protein PMEL
UniProt Protein Name
Melanocyte protein PMEL
UniProt Synonym Protein Names
ME20-M; ME20M
UniProt Gene Name
UniProt Synonym Gene Names
D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S  [Similar Products]
NCBI Summary for gp100
This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]
UniProt Comments for gp100
Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
Product References and Citations for anti-gp100 antibody
Adema, G.J., et al. 1994. J. Biol. Chem. 269: 20126-20133.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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