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anti-GSK3B antibody :: Mouse anti-Human GSK-3 beta Monoclonal Antibody

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Catalog # MBS670122
Unit / Price
  50 Tests  /  $500 +1 FREE 8GB USB
anti-GSK3B antibody
Product Name

GSK-3 beta (GSK3B), Monoclonal Antibody

Also Known As

Mouse Anti-Human GSK-3b-SEPH

Product Synonym Names
Mouse Anti-Human GSK-3b - Sepharose-Labeled; Mouse Anti-Human GSK-3 beta
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
420
Chromosome Location
Chromosome: 3; NC_000003.11 (119540800..119813264, complement). Location: 3q13.3
OMIM
605004
3D Structure
ModBase 3D Structure for P49841
Clonality
Monoclonal
Isotype
Mouse IgG2b
Clone Number
5D6
Host
Mouse
Species Reactivity
Human
Specificity
Human GSK-3 beta
Glycogen synthase kinase-3 (GSK-3) is a protein serine kinase that phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK-3 and subsequently inhibits GSK-3 acutely leading to the stimulation of glycogen synthesis. GSK-3 signaling is performed by two isoforms, GSK-3 alpha and GSK-3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK-3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.
Form/Format
Sepharose 4B (SEPH) Conjugate
Characterization
To insure lot-to-lot consistency, each batch of product is tested by western blotting to conform with the characteristics of a standard reference reagent.
Preparation and Storage
The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The Sepharose 4B (SEPH) conjugate is supplied as 50 tests in 2 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. Shake well before use.
Reagents are stable for the period shown on the label if stored as directed.
Other Notes
Small volumes of anti-GSK3B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-GSK3B antibody
ELISA; Immunoprecipitation; Western Blot
Application Notes for anti-GSK3B antibody
Immunoprecipitation Suggested Dilution: 40uL
NCBI/Uniprot data below describe general gene information for GSK3B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,744 Da[Similar Products]
NCBI Official Full Name
glycogen synthase kinase-3 beta isoform 2
NCBI Official Synonym Full Names
glycogen synthase kinase 3 beta
NCBI Official Symbol
GSK3B  [Similar Products]
NCBI Official Synonym Symbols
GSK3B
  [Similar Products]
NCBI Protein Information
glycogen synthase kinase-3 beta; GSK-3 beta; GSK3beta isoform; OTTHUMP00000165239; OTTHUMP00000165240
UniProt Protein Name
Glycogen synthase kinase-3 beta
Protein Family
UniProt Gene Name
GSK3B  [Similar Products]
UniProt Entry Name
GSK3B_HUMAN
NCBI Summary for GSK3B
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Comments for GSK3B
Function: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. May be required for early embryo development and neuron differentiation

By similarity. Ref.7 Ref.11 Ref.30 Ref.34

Catalytic activity: ATP + [tau protein] = ADP + [tau protein] phosphate.

Enzyme regulation: Activated by phosphorylation at Tyr-216. Inhibited when phosphorylated by AKT1. Ref.34

Subunit structure: Monomer

By similarity. Interacts with ARRB2 and DISC1

By similarity. Interacts with CABYR, MMP2, MUC1, NIN and PRUNE Interacts with AXIN1; the interaction mediates hyperphosphorylates of CTNNB1 leading to its ubiquitination and destruction. Interacts with and phosphorylates SNAI1. Interacts with DNM1L (via a C-terminal domain). Found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B

By similarity. Ref.6 Ref.7 Ref.9 Ref.10 Ref.11 Ref.13 Ref.18 Ref.20 Ref.27 Ref.34

Subcellular location: Cytoplasm. Nucleus. Cell membrane. Note: The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. Ref.11 Ref.30

Tissue specificity: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.

Post-translational modification: Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. Ref.9 Ref.12 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.28 Ref.29 Ref.30 Ref.34

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.Contains 1 protein kinase domain.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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