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anti-GST antibody :: Mouse anti-Human GST Monoclonal Antibody

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Catalog # MBS200015
Unit / Price
  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Product Name

GST, Monoclonal Antibody

Also Known As

GST antibody

Product Synonym Names
Glutathione S-Transferase; GST; GST 26; Sj26 antigen; SjGST
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
1E5
Host
Mouse
Species Reactivity
Human
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant Glutathione S transferase (GST) purified from E. coli
Clone Note
Anti-GST mAb, clone 1E5, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant GST protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-GST antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GST antibody
Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GST proteins can be purified by immobilized glutathione affinity chromatography. Many recombinant proteins have been engineered with GST tags to facilitate the detection, isolation and purification of these proteins. Monoclonal antibody reacting specifically with GST may be useful in various immunotechnique, to identify the expression of a GST fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a GST fusion protein expressing vectors
Product Categories/Family for anti-GST antibody
Applications Tested/Suitable for anti-GST antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-GST antibody
WB: 1:500 ~ 2,000.
Recommended starting dilution is 1:1,000.

Western Blot (WB) of anti-GST antibody
Recombinant GST (28kDa) and GST-fusion protein (61kDa) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GST antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Arrows indicate GST and GST-fusion proteins (20ng).
anti-GST antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-GST antibody
ICC/IF analysis of GST in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GST antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
anti-GST antibody Immunofluorescence (IF) image
Protein Family
Product References and Citations for anti-GST antibody
Smith, D., et al., Proc. Natl. Acad. Sci. USA, 83, 8703 (1986).; Cartwright, G., et al., J. Immunol. Meth., 179, 31 (1995).; Hibma, M., et al., Nucl. Ac. Res., 22, 3806 (1994).

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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