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anti-HAX1 antibody :: Mouse anti-Human HAX1 Monoclonal Antibody

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Catalog # MBS200151
Unit / Price
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  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Product Name

HAX1, Monoclonal Antibody

Full Product Name

HAX1 antibody

Product Synonym Names
HCLS1 associated protein X-1; HS1BP1; HCLSBP1; HCLS1 (and PKD2) associated protein
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
U68566 mRNA
3D Structure
ModBase 3D Structure for O00165
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT3C5
Host
Mouse
Species Reactivity
Human
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human HAX1 (1-279aa) purified from E. coli
Clone Note
Anti-human HAX1 mAb, clone AT3C5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HAX1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-HAX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HAX1 antibody
HAX1 is known to associate with hematopoietic cell-specific Lyn substrate 1 (HS1), one of the substrates of receptor-coupled tyrosine kinases activated during clonal expansion and deletion in lymphoid cells. It also interacts with the product of the polycystic kidney disease 2 (PKD2) gene and with the F-actin-binding protein, cortactin. HAX1 is also reported to bind to hairpin structures in vimentin and DNA polymerase beta mRNAs, so may play a role in mRNA stability and transport. It may also function in promoting cell survival. Defects in HAX1 are the cause of autosomal recessive severe congenital neutropenia 3 (SCN3) also called Kostmann disease.
Product Categories/Family for anti-HAX1 antibody
Applications Tested/Suitable for anti-HAX1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-HAX1 antibody
WB: 1:1000 ~ 2000.
Recommended starting dilution is 1:1000.

Western Blot (WB) of anti-HAX1 antibody
The HeLa cell lysate (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HAX1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HAX1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Raji cell lysate
anti-HAX1 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-HAX1 antibody
ICC/IF analysis of HAX1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HAX1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
anti-HAX1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for HAX1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,801 Da
NCBI Official Full Name
HCLS1-associated protein X-1 isoform a
NCBI Official Synonym Full Names
HCLS1 associated protein X-1
NCBI Official Symbol
NCBI Official Synonym Symbols
SCN3; HS1BP1; HCLSBP1
  [Similar Products]
NCBI Protein Information
HCLS1-associated protein X-1
UniProt Protein Name
HCLS1-associated protein X-1
UniProt Synonym Protein Names
HS1-associating protein X-1; HAX-1; HS1-binding protein 1; HSP1BP-1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
HS1BP1; HAX-1; HSP1BP-1  [Similar Products]
NCBI Summary for HAX1
The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for HAX1
Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex (PubMed:26997484). Slows down the rate of inactivation of KCNC3 channels (PubMed:26997484). Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.
Product References and Citations for anti-HAX1 antibody
Klein C, et al., (2007) Nat Genet. 39 (1):86-92; Yin H, et al., (2001) Cytokine. 15 (3):122-37

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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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