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anti-LOC541815 antibody :: Mouse anti-Human Hemoglobin Monoclonal Antibody

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Catalog # MBS249145
Unit / Price
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  0.25 mg  /  $530 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

Hemoglobin (LOC541815), Monoclonal Antibody

Popular Item
Full Product Name

Mouse Monoclonal [clone M1709Hg2] (IgG1) to Human Hemoglobin

Product Synonym Names
Anti-Hemoglobin Antibody (clone M1709Hg2) IHC-plus; Hemoglobin; Human Hemoglobin
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M1709Hg2
Host
Mouse
Species Reactivity
Human
Specificity
Human Hemoglobin
Purity/Purification
Ion exchange chromatography
Form/Format
50 mM NaCl, 10mM PBS (pH 8.0) with 0.1% sodium azide
Concentration
1 mg/ml (lot specific)
Immunogen Species
Hemoglobin antibody was raised against Human
Immunogen Description
Human hemoglobin.
Target
hemoglobin
Antigen Type
Purified protein
Immunogen
Hemoglobin antibody was raised against human hemoglobin.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Other Notes
Small volumes of anti-LOC541815 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-LOC541815 antibody
Immunohistochemistry (IHC - Paraffin), ELISA (EIA), LateralFlow
Application Notes for anti-LOC541815 antibody
ELISA, IHC-P (10 ug/ml), LateralFlow
Optimal dilution to be determined by the researcher.

Immunohistochemistry (IHC) of anti-LOC541815 antibody
Anti-Hemoglobin antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-LOC541815 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for LOC541815. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
hemoglobin
NCBI Official Symbol
LOC541815  [Similar Products]
NCBI Protein Information
hemoglobin; GRMZM2G067402; non-symbiotic hemoglobin; reversible oxygen binding
Protein Family
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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