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anti-HPA antibody :: Mouse anti-Human Heparanase (HPA) Monoclonal Antibody

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Catalog # MBS2090630
Unit / Price
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $110 +1 FREE 8GB USB
  0.05 mg  /  $140 +1 FREE 8GB USB
  0.1 mg  /  $175 +1 FREE 8GB USB
  0.2 mg  /  $225 +1 FREE 8GB USB
  1 mg  /  $510 +1 FREE 8GB USB
Product Name

Heparanase (HPA), Monoclonal Antibody

Also Known As

Monoclonal Antibody to Heparanase (HPA)

Product Synonym Names
HPSE; HPR1; HPSE1; HSE1; Endo-glucoronidase;
Product Gene Name
Matching Pairs
Unconjugated Antibody: Heparanase (HPA) Clone #C1 (MBS2090630)
Biotin Conjugated Secondary Antibody: IgG (MBS2086030)
Matching Pairs
Unconjugated Antibody: Heparanase (HPA) Clone #C1 (MBS2090630)
FITC Conjugated Secondary Antibody: IgG (MBS2086031)
Matching Pairs
Unconjugated Antibody: Heparanase (HPA) Clone #C1 (MBS2090630)
Biotin Conjugated Secondary Antibody: IgG (MBS2086033)
Matching Pairs
Unconjugated Antibody: Heparanase (HPA) Clone #C1 (MBS2090630)
FITC Conjugated Secondary Antibody: IgG (MBS2086034)
Matching Pairs
Unconjugated Antibody: Heparanase (HPA) Clone #C1 (MBS2090630)
HRP Conjugated Secondary Antibody: IgG (MBS2086035)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
543
OMIM
AF152376 mRNA
3D Structure
ModBase 3D Structure for Q9Y251
Clonality
Monoclonal
Isotype
IgG2a Lambda
Clone Number
C1
Host
Mouse
Species Reactivity
Human
Specificity
The antibody is a mouse monoclonal antibody raised against HPA. It has been selected for its ability to recognize HPA in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid
Concentration
1mg/mL (lot specific)
Immunogen
Recombinant Heparanase (HPA)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of anti-HPA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HPA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes for anti-HPA antibody
Western Blot: 0.5-5ug/mL
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL

Western Blot (WB) of anti-HPA antibody
Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 2ug/ml Mouse Anti-Human HPA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
anti-HPA antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-HPA antibody
Western Blot: Sample: Recombinant HPA, Human.
anti-HPA antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for HPA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,791 Da
NCBI Official Full Name
heparanase isoform 1 preproprotein
NCBI Official Synonym Full Names
heparanase
NCBI Official Symbol
NCBI Official Synonym Symbols
HPA; HPA1; HPR1; HSE1; HPSE1
  [Similar Products]
NCBI Protein Information
heparanase
UniProt Protein Name
Heparanase
UniProt Synonym Protein Names
Endo-glucoronidase; Heparanase-1; Hpa1
UniProt Gene Name
UniProt Synonym Gene Names
HEP; HPA; HPA1; HPR1; HPSE1; HSE1; Hpa1  [Similar Products]
NCBI Summary for HPA
Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Comments for HPA
HPSE: Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up- regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis. Belongs to the glycosyl hydrolase 79 family.

Protein type: EC 3.2.1.166; Extracellular matrix; Glycan Metabolism - glycosaminoglycan degradation; Hydrolase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21.23

Cellular Component: extracellular region; intracellular membrane-bound organelle; lysosomal lumen; lysosomal membrane; lysosome; membrane raft; nucleoplasm; nucleus; proteinaceous extracellular matrix

Molecular Function: beta-glucuronidase activity; heparanase activity; protein binding; protein dimerization activity; syndecan binding

Biological Process: angiogenesis involved in wound healing; cell-matrix adhesion; glycosaminoglycan catabolic process; heparan sulfate proteoglycan catabolic process; neutrophil degranulation; positive regulation of blood coagulation; positive regulation of hair follicle development; positive regulation of osteoblast proliferation; positive regulation of protein kinase B signaling; proteoglycan metabolic process; regulation of hair follicle development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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