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anti-HK2 antibody :: Mouse anti-Human Hexokinase 1 Monoclonal Antibody

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Catalog # MBS200075
Unit / Price
  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Hexokinase 1 (HK2), Monoclonal Antibody

Also Known As

Hexokinase 1, 2 antibody

Product Synonym Names
HK2; Hexokinase 2; EC 2.7.1.1; Hexokinase type II; HK II; Muscle form hexokinase; Hexokinase-2; hexokinase 2,; HK1; Hexokinase 1; EC 2.7.1.1; Hexokinase type I; HK I; Brain form hexokinase; Hexokinase-1; BB404130; dea; Glycolytic enzyme; HEXOKIN; Hexokinase PI; Hexokinase type I; Hexokinase; tumor isozyme; Hexokinase-A; HK1 tb; Hk1-s; HK1-ta; HK1-tc; HKI; HXK1; mHk1-s.
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
917
OMIM
Z46376 mRNA
3D Structure
ModBase 3D Structure for P52789
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
1A7
Host
Mouse
Species Reactivity
Human
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human Hexokinase 2 (1-917aa) purified from E. coli
Clone Note
Anti-human Hexokinase 2 mAb, clone 1A7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hexokinase 2 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-HK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HK2 antibody
Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.
Product Categories/Family for anti-HK2 antibody
Applications Tested/Suitable for anti-HK2 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-HK2 antibody
The antibody recognizes all four isoforms of Hexokinase (1, 2) in recombinant protein.WB: 1:1,000 ~ 3,000.
Recommended starting dilution is 1:2,000.

Western Blot (WB) of anti-HK2 antibody
The Recombinant Proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase I, II antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Hexokinase 1 recombinant protein Lane 2.: Hexokinase 2 recombinant protein Lane 3.: Hexokinase 3 recombinant protein Lane 4.: Hexokinase 4 recombinant protein The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase I, II antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: K562 cell lysate Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection
anti-HK2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for HK2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
102,380 Da[Similar Products]
NCBI Official Full Name
hexokinase-2
NCBI Official Synonym Full Names
hexokinase 2
NCBI Official Symbol
NCBI Official Synonym Symbols
HKII; HXK2
  [Similar Products]
NCBI Protein Information
hexokinase-2
UniProt Protein Name
Hexokinase-2
UniProt Synonym Protein Names
Hexokinase type II; HK II; Muscle form hexokinase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
HK II  [Similar Products]
NCBI Summary for HK2
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]
UniProt Comments for HK2
MiscellaneousIn vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).
Product References and Citations for anti-HK2 antibody
Furuta H, et al., (1996) Genomics 36 (1):206-209; Jon E, et al., (2003) J Exp Biology 206:2049-2057

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-HK2 antibody
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