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Mouse anti-Human HLA Class 1 Antigen A2,B17(B57,B58) Monoclonal Antibody

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Catalog # MBS604130
Unit / Price
  0.1 mL  /  $405 +1 FREE 8GB USB
anti-HLA Class 1 Antigen A2,B17(B57,B58) antibody
Product Name

HLA Class 1 Antigen A2,B17(B57,B58), Monoclonal Antibody

Popular Item
Full Product Name

HLA Class 1 Antigen A2,B17(B57,B58)

Product Synonym Names
Anti -HLA Class 1 Antigen A2,B17(B57,B58)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Monoclonal
Isotype
IgM
Clone Number
4i84
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes human HLA Class 1 Antigen-A2,B57. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
Purity/Purification
Ascites
Form/Format
Supplied as a lyophilized powder. Reconstitute to 100ul with sterile ddH2O to make a 10X stock solution.
Immunogen
B cells expressing HLA class I antigen
Cytotoxicity
Expected Results:

Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Limitations:

Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
Preparation and Storage
Lyophilized powder may be stored at -20°C . Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C . For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-HLA Class 1 Antigen A2,B17(B57,B58) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HLA Class 1 Antigen A2,B17(B57,B58) antibody
This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.
Dilution: Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of 10X working dilution.

Optimal dilution to be determined by the researcher.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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