• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-HLA-A antibody :: Mouse anti-Human HLA Class 2 Antigen DR11 Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS603993
Unit / Price
Scan QR to view Datasheet
  0.1 mL  /  $530 +1 FREE 8GB USB
anti-HLA-A antibody
Product Name

HLA Class 2 Antigen DR11 (HLA-A), Monoclonal Antibody

Popular Item
Full Product Name

HLA Class 2 Antigen DR11 (R0.82)

Product Synonym Names
Anti -HLA Class 2 Antigen DR11 (R0.82)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 6; NC_000006.11 (29910247..29913661). Location: 6p21.3
3D Structure
ModBase 3D Structure for Q9UQU0
Clonality
Monoclonal
Isotype
IgG
Clone Number
4i131
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes HLA Class 2 Antigen-DR11. Specificity was determined at a 1:10 dilution by the Microcytotoxicity test under standard NIH conditions.
Purity/Purification
Ascites
Form/Format
Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 100ul sterile dH2O to make a 10X stock solution.
Grade
Ascites
Source
Ascites fluid
Cytotoxicity
Expected Results:
Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Limitations:
Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
mportant Note for small quantities
Liquids: Apparent volume may be reduced due to evaporation. Add buffer to nominal volume and dilute per instructions. During shipment, small volume products may have adhered to the interior surface of the vial. To recover the maximum amount of product, centrifuge the vial lightly for 1-3 minutes and use a fine tipped pipette for removal. To recover the maximum amount of total product, wash the vial with your assay buffer/diluent. Small volumes of product (less than or equal to 25ul) should be diluted for complete product recovery.
Powders: During shipment, small volume products may have adhered to the interior surface of the vial. To recover the maximum amount of product, centrifuge the vial lightly. To recover the maximum amount of total product, wash the vial with your assay buffer/diluent. Small volumes ~25-50ul should be diluted for complete product recovery.
Preparation and Storage
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-HLA-A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HLA-A antibody
Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Product Categories/Family for anti-HLA-A antibody
Applications Tested/Suitable for anti-HLA-A antibody
This antibody has not been tested in methodologies other than Microcytoxicity Test. Potential applications include Flow Cytometry, Cell typing, Tissue Staining and Chimerism Studies.
Application Notes for anti-HLA-A antibody
Recommended Dilution:
Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of 10X working dilution.
Optimal dilutions to be determined by the researcher.
NCBI/Uniprot data below describe general gene information for HLA-A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
human leucocyte antigen A alpha chain
NCBI Official Synonym Full Names
major histocompatibility complex, class I, A
NCBI Official Symbol
HLA-A  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain
UniProt Protein Name
Leucocyte antigen A alpha chain
UniProt Gene Name
HLA-A  [Similar Products]
UniProt Entry Name
Q9UQU0_HUMAN
NCBI Summary for HLA-A
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]
UniProt Comments for HLA-A
HLAA: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; early endosome membrane; integral to plasma membrane; endoplasmic reticulum; plasma membrane; MHC class I protein complex; Golgi medial cisterna

Molecular Function: protein binding; peptide antigen binding; beta-2-microglobulin binding; T cell receptor binding; TAP binding; receptor binding

Biological Process: regulation of immune response; viral reproduction; detection of bacterium; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; protection from natural killer cell mediated cytotoxicity; positive regulation of interferon-gamma production; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response

Disease: Severe Cutaneous Adverse Reaction, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000