• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-HLA-DP/-DQ/-DR antibody :: Mouse HLA-DP/-DQ/-DR Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS4380017
Unit / Price
Scan QR to view Datasheet
  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
Product Name

HLA-DP/-DQ/-DR, Monoclonal Antibody

Popular Item
Full Product Name

HLA-DP/-DQ/-DR (MHC II)

Product Synonym Names
HLA-DPB1; HLA-DQA1; HLA-DQB1; HLA-DRA; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5;
Product Synonym Gene Name
HLA-DP; HLA-DQ; HLA-DR[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P04440
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CR3/43
Host
Mouse
Species Reactivity
Human. Others not known.
Specificity
Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an  chain (36kDa) and a  chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six  and ten  chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Immunogen
Cells from human tonsil
Cellular Localization
Cell Surface
Hu-chromosome Location
6p21.3 (HLA-DP & HLA-DR)
Positive Control
Raji cells. Tonsil or lymph node
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-HLA-DP/-DQ/-DR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HLA-DP/-DQ/-DR antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-HLA-DP/-DQ/-DR antibody
IF: 0.5-1ug/ml
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-HLA-DP/-DQ/-DR antibody
Formalin-fixed, paraffin-embedded human Tonsil stained with HLA-DP/-DQ/-DR Monoclonal Antibody (CR3/43).
anti-HLA-DP/-DQ/-DR antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HLA-DP/-DQ/-DR antibody
Formalin-fixed, paraffin-embedded human Tonsil stained with HLA-DP/-DQ/-DR Monoclonal Antibody (CR3/43).
anti-HLA-DP/-DQ/-DR antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for HLA-DP/-DQ/-DR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
36kDa (alpha chain); 27kDa (beta chain)
NCBI Official Full Name
HLA class II histocompatibility antigen, DP beta 1 chain
NCBI Official Synonym Full Names
major histocompatibility complex, class II, DP beta 1
NCBI Official Symbol
HLA-DPB1  [Similar Products]
NCBI Official Synonym Symbols
DPB1; HLA-DP; HLA-DPB; HLA-DP1B
  [Similar Products]
NCBI Protein Information
HLA class II histocompatibility antigen, DP beta 1 chain
UniProt Protein Name
HLA class II histocompatibility antigen, DP beta 1 chain
UniProt Synonym Protein Names
HLA class II histocompatibility antigen, DP(W4) beta chain; MHC class II antigen DPB1
UniProt Gene Name
HLA-DPB1  [Similar Products]
UniProt Synonym Gene Names
HLA-DP1B  [Similar Products]
NCBI Summary for HLA-DP/-DQ/-DR
HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq, Jul 2008]
UniProt Comments for HLA-DP/-DQ/-DR
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
Product References and Citations for anti-HLA-DP/-DQ/-DR antibody
Gatter et al. 1982. J Clin Pathol. 35(11):1253-67. Gatter et al. 1982. Semin Oncol. 9(4):517-25.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000