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anti-HLA-DR antibody :: Rat anti-Human HLA-DR Monoclonal Antibody

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Catalog # MBS520340
Unit / Price
  0.1 mg  /  $235 +1 FREE 8GB USB
Testing Data
Product Name

HLA-DR, Monoclonal Antibody

Popular Item
Also Known As

Anti-Human HLA-DR, Biotin, (Clone YE2/36 HLK) (rat IgG2a)

Product Synonym Names
HLA-DR, Biotin, (Clone YE2/36 HLK) (rat IgG2a); Purified Anti-Human HLA-DR Monoclonal Antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
42
Chromosome Location
Chromosome: 6; NC_000006.11 (32627241..32634466, complement). Location: 6p21.3
3D Structure
ModBase 3D Structure for Q29900
Clonality
Monoclonal
Isotype
Rat IgG2a
Clone Number
YE2/36 HLK
Host
Rat
Species Reactivity
Human
Specificity
HLA-DR (HLA-DR Ia alpha chain, cross reactive with mouse H-2, I-A sub-region)
Form/Format
Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascites via Protein G Chromatography)
Concentration
Antibody Concentration: 0.1 mg/ml (lot specific)
Immunogen
EHR-B Ramos cells
Donor
immunized AO rat spleen cells
Fusion Partner
Y3 Ag1.2.3 rat myeloma
Presentation
100ug Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Preparation and Storage
Store at 4 degree C. For long term storage, aliquot and freeze unused portions at -20 degree C, in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-HLA-DR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HLA-DR antibody
purified anti-human HLA-DR monoclonal antibody recognizes an epitope on the Ia alpha chain of the Human lymphocyte antigen. This antibody has also been shown to recognize the H-2, I-A region of the mouse.
Applications Tested/Suitable for anti-HLA-DR antibody
Immunohistochemistry (IHC)
Application Notes for anti-HLA-DR antibody
flow cytometry, immunofluorescence1 and immunohistochemistry3 (frozen and paraffin sections).

Testing Data of anti-HLA-DR antibody
anti-HLA-DR antibody Testing Data image
NCBI/Uniprot data below describe general gene information for HLA-DR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
5,068 Da
NCBI Official Full Name
HLA-DR
NCBI Official Synonym Full Names
major histocompatibility complex, class II, DQ beta 1
NCBI Official Symbol
HLA-DQB1  [Similar Products]
NCBI Official Synonym Symbols
IDDM1; CELIAC1; HLA-DQB
  [Similar Products]
NCBI Protein Information
HLA class II histocompatibility antigen, DQ beta 1 chain; MHC DQ beta; MHC class2 antigen; lymphocyte antigen; MHC class II antigen DQB1; MHC class II DQ beta chain; MHC class II antigen HLA-DQ-beta-1; MHC class II HLA-DQ beta glycoprotein
UniProt Protein Name
HLA-DR
UniProt Entry Name
Q29900_HUMAN
NCBI Summary for HLA-DR
HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
UniProt Comments for HLA-DR
HLA-DQB1: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; membrane; lysosomal membrane; plasma membrane; trans-Golgi network membrane; endosome membrane; MHC class II protein complex

Molecular Function: MHC class II receptor activity; peptide antigen binding

Biological Process: humoral immune response mediated by circulating immunoglobulin; cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; immunoglobulin production during immune response; T cell receptor signaling pathway

Disease: Celiac Disease; Creutzfeldt-jakob Disease; Multiple Sclerosis, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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