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anti-HP antibody :: Mouse anti-Human HP / Haptoglobin Monoclonal Antibody

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Catalog # MBS245473
Unit / Price
  0.05 mL  /  $530 +1 FREE 8GB USB
Product Name

HP / Haptoglobin, Monoclonal Antibody

Popular Item
Also Known As

Mouse Monoclonal [clone 2F4] (IgG1,k) to Human HP / Haptoglobin

Product Synonym Names
Anti-HP / Haptoglobin Antibody (clone 2F4) IHC-plus; HP; Binding peptide; Haptoglobin alpha(1S)-beta; Haptoglobin; beta polypeptide; Haptoglobin; Haptoglobin alpha(2FS)-beta; Haptoglobin; alpha polypeptide; HP2ALPHA2; BP; Zonulin; HPA1S; Human HP; Haptoglobin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
406
OMIM
gene 614081
3D Structure
ModBase 3D Structure for P00738
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2F4
Host
Mouse
Species Reactivity
Human
Purity/Purification
Purified
Form/Format
HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol
Concentration
1 mg/ml (lot specific)
Target
HP/Haptoglobin
Target Species
Human
Immunogen Description
Protein purified from Human plasma.
Immunogen
HP / Haptoglobin antibody was raised against protein purified from Human plasma.
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-HP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HP antibody
The Haptoglobin (HP) gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin.
Applications Tested/Suitable for anti-HP antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-HP antibody
ELISA, IHC-P (1:50), WB (1:2000)
Usage: Immunohistochemistry: MBS245547 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS245547 was determined to be 1:50.

Immunohistochemistry (IHC) of anti-HP antibody
Anti-Haptoglobin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
anti-HP antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HP antibody
Anti-Haptoglobin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
anti-HP antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-HP antibody
 
anti-HP antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for HP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,452 Da
NCBI Official Full Name
haptoglobin isoform 1 preproprotein
NCBI Official Synonym Full Names
haptoglobin
NCBI Official Symbol
NCBI Official Synonym Symbols
BP; HPA1S; HP2ALPHA2
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NCBI Protein Information
haptoglobin; zonulin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, beta polypeptide; haptoglobin, alpha polypeptide
UniProt Protein Name
Haptoglobin
UniProt Synonym Protein Names
ZonulinCleaved into the following 2 chains:Haptoglobin alpha chain; Haptoglobin beta chain
UniProt Gene Name
UniProt Entry Name
HPT_HUMAN
NCBI Summary for HP
This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]
UniProt Comments for HP
HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: extracellular space; extracellular region

Molecular Function: antioxidant activity; protein binding; hemoglobin binding; catalytic activity

Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; metabolic process; immune system process; defense response to bacterium; negative regulation of oxidoreductase activity; acute-phase response; defense response

Disease: Anhaptoglobinemia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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