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anti-HSP60 antibody :: Mouse anti-Human HSP60 Monoclonal Antibody

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Catalog # MBS2549941
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Product Name

HSP60, Monoclonal Antibody

Also Known As

HSP60 Monoclonal Antibody

Product Synonym Names
60 kDa heat shock protein; mitochondrial; 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
573
OMIM
M22382 mRNA
3D Structure
ModBase 3D Structure for P10809
Clonality
Monoclonal
Isotype
IgG
Clone Number
PT0153
Host
Mouse
Species Reactivity
Human
Specificity
This antibody detects endogenous levels of HSP60
Purity/Purification
Antigen Affinity Purification
Concentration
1mg/mL (lot specific)
Function
Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Immunogen
Full length protein from eukaryotic expression system.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Conjugation
Unconjugated
Positive Control
Colon cancer, Liver cancer
Cellular Localization
Mitochondrion
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-HSP60 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HSP60 antibody
HSPs is a group of stress protein cells induced by stress stimulation, and it is related to tumorigenesis, proliferation and differentiation. HSPs can be classified into 3 types according to their different molecular weight, and the distribution and function of each type of HSPs are different. HSP60 is mainly used in research of tongue squamous cell carcinoma, colon cancer, pancreatic cancer and breast cancer, which may have prognostic significance.
Product Categories/Family for anti-HSP60 antibody
Applications Tested/Suitable for anti-HSP60 antibody
Immunohistochemistry (IHC) Paraffin (IHC-p)
Application Notes for anti-HSP60 antibody
IHC: 1:200-1:300

Immunohistochemistry (IHC) of anti-HSP60 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver cancer tissue labeling HSP60 Monoclonal Antibody with E-PA-3670 at dilution of 1:200. Heat induced epitope retrieval was performed by boiling the tissue sections in citrate buffer(PH6.0)for 20 minutes. Incubation with the primary antibody was performed for overnight at 4 degree C followed by keeping at room temperature for 15mins. HRP conjugated Anti-Mouse secondary antibody was applied for 30 minutes at room temperature followed by DAB as chromogen, thencounterstainwithhaematoxylin.
anti-HSP60 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HSP60 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon cancer tissue labeling HSP60 Monoclonal Antibody with E-PA-3670 at dilution of 1:200. Heat induced epitope retrieval was performed by boiling the tissue sections in citrate buffer(PH6.0)for 20 minutes. Incubation with the primary antibody was performed for overnight at 4 degree C followed by keeping at room temperature for 15mins. HRP conjugated Anti-Mouse secondary antibody was applied for 30 minutes at room temperature followed by DAB as chromogen, thencounterstainwithhaematoxylin.
anti-HSP60 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for HSP60. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,100 Da
NCBI Official Full Name
60 kDa heat shock protein, mitochondrial
NCBI Official Synonym Full Names
heat shock protein family D (Hsp60) member 1
NCBI Official Symbol
HSPD1  [Similar Products]
NCBI Official Synonym Symbols
HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
  [Similar Products]
NCBI Protein Information
60 kDa heat shock protein, mitochondrial
UniProt Protein Name
60 kDa heat shock protein, mitochondrial
UniProt Synonym Protein Names
60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein
Protein Family
UniProt Gene Name
HSPD1  [Similar Products]
UniProt Synonym Gene Names
HSP60; CPN60; HSP-60; Hsp60  [Similar Products]
NCBI Summary for HSP60
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
UniProt Comments for HSP60
HSP60: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Interacts with HRAS. Interacts with HBV protein X and HTLV-1 protein p40tax. Belongs to the chaperonin (HSP60) family.

Protein type: Chaperone; Mitochondrial

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: cell surface; clathrin-coated pit; cyclin-dependent protein kinase activating kinase holoenzyme complex; cytoplasm; cytosol; early endosome; extracellular exosome; extracellular matrix; extracellular space; lipopolysaccharide receptor complex; membrane; mitochondrial inner membrane; mitochondrial matrix; mitochondrion; myelin sheath; plasma membrane; protein complex; secretory granule

Molecular Function: apolipoprotein A-I binding; apolipoprotein binding; ATP binding; ATPase activity; chaperone binding; DNA replication origin binding; double-stranded RNA binding; enzyme binding; high-density lipoprotein particle binding; lipopolysaccharide binding; p53 binding; protein binding; RNA binding; single-stranded DNA binding; ubiquitin protein ligase binding; unfolded protein binding

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; adhesion of symbiont to host; apoptotic mitochondrial changes; B cell activation; B cell cytokine production; B cell proliferation; chaperone-mediated protein complex assembly; de novo'protein folding; interaction with symbiont; isotype switching to IgG isotypes; MyD88-dependent toll-like receptor signaling pathway; negative regulation of apoptosis; positive regulation of apoptosis; positive regulation of interferon-alpha production; positive regulation of interferon-gamma production; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of interleukin-6 production; positive regulation of macrophage activation; positive regulation of T cell activation; positive regulation of T cell mediated immune response to tumor cell; protein import into mitochondrial intermembrane space; protein maturation; protein refolding; protein stabilization; regulation of transcription from RNA polymerase II promoter; response to cold; response to unfolded protein; T cell activation; viral process

Disease: Leukodystrophy, Hypomyelinating, 4; Spastic Paraplegia 13, Autosomal Dominant
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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