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anti-Hsp90 antibody :: Mouse anti-Human, Hsp90 Monoclonal Antibody

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Catalog # MBS200047
Unit / Price
  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Product Name

Hsp90, Monoclonal Antibody

Popular Item
Also Known As

Hsp90 antibody

Product Synonym Names
Heat shock protein HSP 90-alpha; HSP 86; Renal carcinoma antigen NY-REN-38; HSPC1; HSPCA; Heat shock protein 90-alpha Renal carcinoma antigen NY REN 38; D6S182; FLJ26984; FLJ31884; Heat shock 90kDa protein 1 alpha; heat shock protein 90kDa alpha (cytosolic); class A member 2; HSP84; HSP86; Hsp89; HSP89A; Hsp90; HSP90A; HSP90AA1; HSP90ALPHA; HSP90N; HSPCAL3; HSPCB; HSPN; LAP2; Lipopolysaccharide associated protein2; LPS associated protein 2; NY REN 38 antigen; hsp90; Heat shock protein HSP 90-alpha
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
732
OMIM
X15183 mRNA
3D Structure
ModBase 3D Structure for P07900
Clonality
Monoclonal
Isotype
Mouse IgG2bheavy chain and k light chain
Clone Number
4F10
Host
Mouse
Species Reactivity
Human,
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human Hsp90 (1-732aa) purified from E. coli
Clone Note
Anti-human Hsp90 mAb, clone 4F10, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp90 protein.
Preparation and Storage
Can be stored at 4°C for short term ( 1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C . Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-Hsp90 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Hsp90 antibody
Hsp90 is a human heat shock protein. In response to adverse change in their environment, cell from all organisms increase the expression of a class of proteins referred to as heat shock or stress protein. The Hsp90, a highly conserved stress-induced protein, is abundantly expressed in most tissues under non-stress conditions and is required for eukaryotic cell viability. Hsp90 is primarily a cytoplasmic protein and its function remains unknown. It exists in a dimeric form and has been observed to bind to several other cellular proteins such as retro-virus kinases, steroid receptor, heme-regulated protein kinase, actin and tubulin.
Product Categories/Family for anti-Hsp90 antibody
Applications Tested/Suitable for anti-Hsp90 antibody
ELISA (EIA), WB (Cell lysate), Immunohistochemistry (IHC)
Application Notes for anti-Hsp90 antibody
The antibody has been tested by ELISA, Western Blot, and Immunohistochemistry analysis to assure specifity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended diltuion range for Western Blot is 1:500~ 1:200 and immunohistochemistry analysis is 1:100~200. Recommended starting dilution for Western Blot is 1:1000 and Immunohistochemistry is 1:100.

Western Blot (WB) of anti-Hsp90 antibody
The Recombinant Human HSP90 (50ng) and Cell lysates(40ug)
were resolved by SDS-PAGE, transferred to PVDF membrane
and probed with anti-human HSP90antibody (1:1000). Proteins
were visualized using a goat anti-mouse secondary antibody
conjugated to HRP and an ECL detection system
Lane 1. : Hsp90 recombinant protein
Lane 2. : 293T cell lysate
Lane 3. : HeLa cell lysate
Lane 4. : HepG2 cell lysate
anti-Hsp90 antibody Western Blot (WB) (WB) image
Flow Cytometry (FC) of anti-Hsp90 antibody
Flow cytometry analysis of HSP90in HepG2cell line,staining at 2-5ug for 1x106cellsredline). The secondary antibody used goat anti-mouse IgGAlexa fluor 488 conjugate. Isotype control antibody was mouse IgG(black line).
anti-Hsp90 antibody Flow Cytometry (FC) (FC/FACS) image
Immunohistochemistry (IHC) of anti-Hsp90 antibody
Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100)for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detectedusing Diaminobenzidine (DAB)
anti-Hsp90 antibody Immunohistochemistry (IHC) (IHC) image
Testing Data(TD) of anti-Hsp90 antibody
ICC/IF analysis of HSP90in HeLa cells line, stained with DAPI(Blue) for nucleus staining and monoclonal anti-human HSP90 antibody (1: 100) with goat anti-mouse IgG-Alexa fluor 488 conjugate(Green).
anti-Hsp90 antibody Testing Data(TD) image
NCBI/Uniprot data below describe general gene information for Hsp90. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
heat shock protein HSP 90-alpha isoform 2
NCBI Official Synonym Full Names
heat shock protein 90 alpha family class A member 1
NCBI Official Symbol
HSP90AA1  [Similar Products]
NCBI Official Synonym Symbols
EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
  [Similar Products]
NCBI Protein Information
heat shock protein HSP 90-alpha
UniProt Protein Name
Heat shock protein HSP 90-alpha
UniProt Synonym Protein Names
Heat shock 86 kDa; HSP 86; HSP86; Lipopolysaccharide-associated protein 2; LAP-2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38
Protein Family
UniProt Gene Name
HSP90AA1  [Similar Products]
UniProt Synonym Gene Names
HSP90A; HSPC1; HSPCA; HSP 86; HSP86; LAP-2; LPS-associated protein 2  [Similar Products]
NCBI Summary for Hsp90
The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Comments for Hsp90
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed:11274138, PubMed:15577939, PubMed:15937123, PubMed:27353360). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed:27295069, PubMed:26991466). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed:25973397). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed:24613385).
Product References and Citations for anti-Hsp90 antibody
B-T, et al., (1984) Molecular & Cellular Biol 4: 2802-2810; Pritchard, K. A, et al., (2001) J Biol Chem 276:17621-17624; Miyamoto, A, et al., (2002) Nature 416:865-869

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