• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-IGHG4 antibody :: Mouse IgG4 (Ig Heavy Constant Gamma 4) Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS4380034
Unit / Price
Scan QR to view Datasheet
  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
Product Name

IgG4 (Ig Heavy Constant Gamma 4) (IGHG4), Monoclonal Antibody

Popular Item
Full Product Name

IgG4 (Ig Heavy Constant Gamma 4) (G4m Marker)

Product Synonym Names
Ig gamma 4 chain C region; IGHG4; Immunoglobulin heavy constant gamma 4 (G4m marker)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01861
Clonality
Monoclonal
Isotype
IgG1
Clone Number
IGHG4/1345
Host
Mouse
Species Reactivity
Human. Others not known.
Specificity
The regions of relatively constant sequence beyond the variable regions of immunoglobulins are termed constant regions (C regions) and are present in both the heavy and light chains. With very few exceptions, the sites of attachment for carbohydrates on immunoglobulins are located in these C regions. These regions also function to hold the variable regions together by using the disulfide bond between them. The C regions facilitate interaction with the antigen by increasing the maximum rotation of the immunoglobulin arms. Reportedly, a large population of patients with recurrent respiratory tract infection has low IgG4 concentrations. IgG4-related sclerosing disease has been recognized as a systemic disease entity characterized by an elevated serum IgG4 level, sclerosing fibrosis, and diffuse lymphoplasmacytic infiltration with the presence of many IgG4-positive plasma cells. IgG4 is overexpressed in inflammatory pseudotumor (IPT) and under expressed in inflammatory myofibroblastic tumor (IMT). In pulmonary nodular lymphoid hyperplasia (PNLH), there are an increased number of IgG4+ plasma cells.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Immunogen
Recombinant human full-length IGHG4
Cellular Localization
Cytoplasmic
Hu-chromosome Location
14q32.33
Positive Control
Tonsil
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-IGHG4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-IGHG4 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-IGHG4 antibody
FC/FACS: 0.5-1ug/million cells
IF: 0.5-1ug/ml
WB: 0.5-1ug/ml
IHC-F: 0.5-1.0ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-IGHG4 antibody
Formalin-fixed, paraffin-embedded Human Tonsil stained with IgG4 Monoclonal Antibody (IGHG4/1345).
anti-IGHG4 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-IGHG4 antibody
Formalin-fixed, paraffin-embedded human Histiocytoma stained with HLA-DR Monoclonal Antibody (LN-3 + HLA-DRB/1067).
anti-IGHG4 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for IGHG4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
150kDa
NCBI Official Full Name
Immunoglobulin heavy constant gamma 4
NCBI Official Synonym Full Names
immunoglobulin heavy constant gamma 4 (G4m marker)
NCBI Official Symbol
IGHG4  [Similar Products]
UniProt Protein Name
Immunoglobulin heavy constant gamma 4
UniProt Synonym Protein Names
Ig gamma-4 chain C regionCurated
Protein Family
UniProt Gene Name
IGHG4  [Similar Products]
UniProt Comments for IGHG4
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Product References and Citations for anti-IGHG4 antibody
Adetugbo, K., et al. 1978. Evolution of immunoglobulin subclasses. Primary structure of a murine myeloma gamma1 chain. J. Biol. Chem. 253: 6068-6075.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000