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anti-INS antibody :: Mouse anti-Human Insulin Monoclonal Antibody

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Catalog # MBS245251
Unit / Price
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  0.05 mg  /  $530 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

Insulin, Monoclonal Antibody

Full Product Name

Mouse Monoclonal [clone 8E2] (IgG1,k) to Human Insulin

Product Synonym Names
Anti-Insulin Antibody (clone 8E2, HRP) IHC-plus; IRDN; IDDM2; Preproinsulin; ILPR; Insulin; MODY10; Proinsulin; Human Insulin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
phenotype 613370
3D Structure
ModBase 3D Structure for P01308
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
8E2
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes human Insulin. Species cross-reactivity: human proinsulin, bovine insulin (30%) and porcine insulin. Does not cross-react with free C-peptide.
Purity/Purification
Protein A Purified
Form/Format
PBS, pH 7.4, 0.05% Proclin 300
Concentration
1 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Purified human insulin.
Immunogen Type
Purified protein
Immunogen
Insulin antibody was raised against purified human insulin.
Antibody Modification
HRP
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze-thaw cycles and prolonged exposure to light.
Other Notes
Small volumes of anti-INS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-INS antibody
Insulin (INS) is a hormone central to regulating carbohydrate and fat metabolism in the body. Insulin causes cells in the liver, muscle, and fat tissue to take up glucose from the blood, storing it as glycogen in the liver and muscle. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds.
Product Categories/Family for anti-INS antibody
Applications Tested/Suitable for anti-INS antibody
Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
Application Notes for anti-INS antibody
ELISA, IHC-P (10 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-INS antibody
Anti-Insulin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-INS antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for INS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,537 Da
NCBI Official Full Name
insulin preproprotein
NCBI Official Synonym Full Names
insulin
NCBI Official Symbol
NCBI Official Synonym Symbols
IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
  [Similar Products]
NCBI Protein Information
insulin; proinsulin; preproinsulin
UniProt Protein Name
Insulin
Protein Family
UniProt Gene Name
UniProt Entry Name
INS_HUMAN
NCBI Summary for INS
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
UniProt Comments for INS
Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family.

Protein type: Secreted; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; extracellular region

Molecular Function: insulin-like growth factor receptor binding; identical protein binding; protein binding; protease binding; hormone activity; insulin receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of vasodilation; glucose homeostasis; activation of NF-kappaB transcription factor; negative regulation of protein oligomerization; positive regulation of glucose import; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; activation of protein kinase B; positive regulation of nitric-oxide synthase activity; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; regulation of amino acid metabolic process; positive regulation of cell differentiation; negative regulation of protein catabolic process; negative regulation of glycogen catabolic process; wound healing; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; glucose transport; regulation of protein secretion; negative regulation of lipid catabolic process; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; negative regulation of vasodilation; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; alpha-beta T cell activation; negative regulation of fatty acid metabolic process; endocrine pancreas development; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cytokine secretion; positive regulation of cell migration

Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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