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anti-ISG15 antibody :: Mouse anti-Human ISG15 Monoclonal Antibody

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Catalog # MBS200045
Unit / Price
  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Product Name

ISG15, Monoclonal Antibody

Popular Item
Full Product Name

ISG15 antibody

Product Synonym Names
Interferon-stimulated gene 15 kDa ubiquitin-like modifier; GIP2; uCRP; IFI15,ISG15,Interferon-stimulated gene; 15 kDa ubiquitin-like modifier; Interferon-stimulated gene; 15 kDa ubiquitin-like modifier IFI 15; Interferon alpha inducible protein; Interferon induced 15 kDa protein; Interferon induced 17 kDa protein; Interferon induced 17 kDa/15 kDa protein; Interferon Stimulated Gene 15; Interferon stimulated protein 15 kDa; ISG 15; ISG15 ubiquitin like modifier; ubiquitin cross reactive protein precursor.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
M13755 mRNA
3D Structure
ModBase 3D Structure for P05161
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
3E5
Host
Mouse
Species Reactivity
Human
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human ISG15 (1-157aa) purified from E. coli
Clone Note
Anti-human ISG15 mAb, clone 3E5, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ISG15 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-ISG15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ISG15 antibody
ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play important roles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15 is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety of proteins when cells are treated with type I interferon or lipopolysaccharide.
Product Categories/Family for anti-ISG15 antibody
Applications Tested/Suitable for anti-ISG15 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-ISG15 antibody
WB: 1:1,000 ~ 2,000.
Recommended starting dilution is 1:1,000.

Western Blot (WB) of anti-ISG15 antibody
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : MCF7 cell lysate
anti-ISG15 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-ISG15 antibody
ICC/IF analysis of ISG15 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ISG15 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
anti-ISG15 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for ISG15. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,888 Da
NCBI Official Full Name
ubiquitin-like protein ISG15
NCBI Official Synonym Full Names
ISG15 ubiquitin-like modifier
NCBI Official Symbol
ISG15  [Similar Products]
NCBI Official Synonym Symbols
G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
  [Similar Products]
NCBI Protein Information
ubiquitin-like protein ISG15
UniProt Protein Name
Ubiquitin-like protein ISG15
UniProt Synonym Protein Names
Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein; hUCRP
Protein Family
UniProt Gene Name
ISG15  [Similar Products]
UniProt Synonym Gene Names
G1P2; UCRP; IP17; hUCRP  [Similar Products]
NCBI Summary for ISG15
The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]
UniProt Comments for ISG15
Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include IFIT1, MX1/MxA, PPM1B, UBE2L6, UBA7, CHMP5, CHMP2A, CHMP4B and CHMP6. Can also isgylate: EIF2AK2/PKR which results in its activation, DDX58/RIG-I which inhibits its function in antiviral signaling response, EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity, UBE2N and UBE2E1 which negatively regulates their activity, IRF3 which inhibits its ubiquitination and degradation and FLNB which prevents its ability to interact with the upstream activators of the JNK cascade therby inhibiting IFNA-induced JNK signaling. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A, HIV-1 and Ebola virus. Restricts HIV-1 and ebola virus via disruption of viral budding. Inhibits the ubiquitination of HIV-1 Gag and host TSG101 and disrupts their interaction, thereby preventing assembly and release of virions from infected cells. Inhibits Ebola virus budding mediated by the VP40 protein by disrupting ubiquitin ligase activity of NEDD4 and its ability to ubiquitinate VP40. ISGylates influenza A virus NS1 protein which causes a loss of function of the protein and the inhibition of virus replication. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity.
Product References and Citations for anti-ISG15 antibody
Jonathan D et al., (1996) Proc Natl Acad Sci USA 93:211-215; Weiming Y et al., (2001) EMBO J 20 (3):362-371; Chen Z et al., (2005) Proc Natl Acad Sci USA 102 (29):10200-10205

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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