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anti-ITGA6 antibody :: Mouse anti-Human ITA6 Monoclonal Antibody

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Catalog # MBS9200194
Unit / Price
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  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Product Name

ITA6 (ITGA6), Monoclonal Antibody

Popular Item
Full Product Name

ITA6 Antibody

Product Synonym Names
Integrin alpha-6; CD49 antigen-like family member F; VLA-6; CD49f; Integrin alpha-6 heavy chain; Integrin alpha-6 light chain; Processed integrin alpha-6; Alpha6p; ITGA6
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
147556
3D Structure
ModBase 3D Structure for P23229
Clonality
Monoclonal
Isotype
IgG1,kappa
Clone Number
129CT49.6.5
Host
Mouse
Species Reactivity
Human
Specificity
ITA6 recombinant protein is used to produce this monoclonal antibody.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.42 (lot specific)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Cellular Location
Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor
Tissue Location
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ITGA6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ITGA6 antibody
The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene.
Applications Tested/Suitable for anti-ITGA6 antibody
Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry-Paraffin (IHC-P)
Application Notes for anti-ITGA6 antibody
WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50

Western Blot (WB) of anti-ITGA6 antibody
Anti-ITA6 at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 127 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
anti-ITGA6 antibody Western Blot (WB) (WB) image
Immunohistochemistry of anti-ITGA6 antibody
ITA6 Monoclonal Antibody (Cat. #MBS9200194) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conj?gation of the secondary antibody and DAB staining. This data demonstrates the use of the ITA6 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
anti-ITGA6 antibody Immunohistochemistry (IHC) image
Immunofluorescence (IF) of anti-ITGA6 antibody
Confocal immunofluorescent analysis of ITA6 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
anti-ITGA6 antibody Immunofluorescence (IF) image
Flow Cytometry (FC/FACS) of anti-ITGA6 antibody
ITA6 Monoclonal Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
anti-ITGA6 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for ITGA6. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
126606
NCBI Official Full Name
integrin alpha-6 isoform b
NCBI Official Synonym Full Names
integrin, alpha 6
NCBI Official Symbol
ITGA6  [Similar Products]
NCBI Official Synonym Symbols
CD49f; VLA-6; ITGA6B
  [Similar Products]
NCBI Protein Information
integrin alpha-6
UniProt Protein Name
Integrin alpha-6
UniProt Synonym Protein Names
CD49 antigen-like family member F; VLA-6; CD_antigen: CD49f
Protein Family
UniProt Gene Name
ITGA6  [Similar Products]
UniProt Synonym Gene Names
Alpha6p  [Similar Products]
UniProt Entry Name
ITA6_HUMAN
NCBI Summary for ITGA6
The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ITGA6
ITGA6: Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5. Interacts with RAB21. Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Belongs to the integrin alpha chain family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: focal adhesion; cell surface; cell-cell adherens junction; hemidesmosome; basal plasma membrane; plasma membrane; basement membrane; filopodium; external side of plasma membrane

Molecular Function: integrin binding; protein binding; metal ion binding; laminin binding

Biological Process: integrin-mediated signaling pathway; skin development; extracellular matrix organization and biogenesis; filopodium formation; positive regulation of apoptosis; cell-matrix adhesion; cell-substrate adhesion; hemidesmosome assembly; cell-cell adhesion; gut development; cellular response to extracellular stimulus; brown fat cell differentiation; cell-substrate junction assembly; positive regulation of cell-cell adhesion; cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; leukocyte migration; positive regulation of phosphorylation

Disease: Epidermolysis Bullosa Junctionalis With Pyloric Atresia
Product References and Citations for anti-ITGA6 antibody
Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility hot-spot. Johnatty SE, et al. PLoS Genet, 2010 Jul 8. PMID 20628624. Prostate cancer risk-associated variants reported from genome-wide association studies: Meta-analysis and their contribution to genetic Variation. Kim ST, et al. Prostate, 2010 Jun 16. PMID 20564319. alpha6-integrin subunit plays a major role in the proangiogenic properties of endothelial progenitor cells. Bouvard C, et al. Arterioscler Thromb Vasc Biol, 2010 Aug. PMID 20508204. Common polymorphisms in ITGA2, PON1 and THBS2 are associated with coronary atherosclerosis in a candidate gene association study of the Chinese Han population. Wang Y, et al. J Hum Genet, 2010 Aug. PMID 20485444. Usage of heparan sulfate, integrins, and FAK in HPV16 infection. Abban CY, et al. Virology, 2010 Jul 20. PMID 20441998.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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