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anti-Ki-67 antibody :: Mouse Ki-67 Monoclonal Antibody

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Catalog # MBS4380461
Unit / Price
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  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $340 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $340 +1 FREE 8GB USB
Product Name

Ki-67, Monoclonal Antibody

Popular Item
Full Product Name

Ki-67 (Proliferating Cell Marker)

Product Synonym Names
KI-67; Ki67; KI-67 Antigen (KIA); MKI67; Proliferation related Ki-67 antigen
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P46013
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
MKI67/2465
Host
Mouse
Species Reactivity
Human. Others not known.
Specificity
Ki-67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki-67 index and the histo-pathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki-67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki-67 is a good marker of proliferating cell populations.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Immunogen
Recombinant fragment of human Ki67 protein (around aa 2293-2478)
Cellular Localization
Nuclear
Hu-chromosome Location
10q26.2
Positive Control
Any actively proliferating cells. Skin, Tonsil or Lymph Node
Other Notes
Small volumes of anti-Ki-67 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Ki-67 antibody
Immunohistochemistry (IHC) Formalin
Application Notes for anti-Ki-67 antibody
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-Ki-67 antibody
Formalin-fixed, paraffin-embedded human Tonsil stained with Ki67 Mouse Monoclonal Antibody (MKI67/2465).
anti-Ki-67 antibody Immunohistochemistry (IHC) (IHC) image
SDS-PAGE Analysis Purified Ki67 Mouse Monoclonal Antibody (MKI67/2465).Confirmation of Integrity and Purity of Antibody.
anti-Ki-67 antibody SDS-Page image
Testing Data of anti-Ki-67 antibody
Analysis of Protein Array containing more than 19, 000 full-length human proteins using Ki67 Mouse Monoclonal Antibody (MKI67/2465).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
anti-Ki-67 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for Ki-67. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
345kDa; 395kDa
NCBI Official Full Name
proliferation marker protein Ki-67 isoform 2
NCBI Official Synonym Full Names
marker of proliferation Ki-67
NCBI Official Symbol
MKI67  [Similar Products]
NCBI Official Synonym Symbols
KIA; MIB-; MIB-1; PPP1R105
  [Similar Products]
NCBI Protein Information
proliferation marker protein Ki-67
UniProt Protein Name
Proliferation marker protein Ki-67
UniProt Synonym Protein Names
Antigen identified by monoclonal antibody Ki-67
UniProt Gene Name
MKI67  [Similar Products]
UniProt Synonym Gene Names
Antigen KI-67Curated; Antigen Ki67Curated  [Similar Products]
NCBI Summary for Ki-67
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
UniProt Comments for Ki-67
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Product References and Citations for anti-Ki-67 antibody
Gerdes J, et al. 1984. J. Immunol. 133:1710. Schluter C, et al. 1993 J. Cell Biol. 123:513.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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