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anti-IGL antibody :: Mouse Lambda Light Chain Monoclonal Antibody

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Catalog # MBS4380044
Unit / Price
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  0.5 mL  /  $300 +1 FREE 8GB USB
Product Name

Lambda Light Chain (IGL), Monoclonal Antibody

Popular Item
Full Product Name

Lambda Light Chain (B-Cell Marker)

Product Synonym Names
Bence Jones Protein; BJP; IGLC 1/2/3; Mcg Marker; Paraprotein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01701
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
LcN-2
Host
Mouse
Species Reactivity
Human. Others not known.
Specificity
This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Form/Format
Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
Immunogen
Purified human IgG
Cellular Localization
Cell Surface, Cytoplasmic and Secreted
Hu-chromosome Location
22q11.22
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen
Preparation and Storage
Antibody with azide - store at 4 to 8 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-IGL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-IGL antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-IGL antibody
FC/FACS: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood
IF: 1:50-1:100

Immunofluorescence (IF) of anti-IGL antibody
Confocal Immunofluorescent analysis of Ramos cells using AF488-labeled Lambda Light Chain Monoclonal Antibody (LcN-2) (Green). DAPI was used to stain the cell nuclei (blue).
anti-IGL antibody Immunofluorescence (IF) image
Immunofluorescence (IF) of anti-IGL antibody
Confocal Immunofluorescent analysis of Ramos cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control)
anti-IGL antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for IGL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
~22.5kDa
NCBI Official Full Name
Immunoglobulin lambda variable 1-51
UniProt Protein Name
Immunoglobulin lambda variable 1-51
UniProt Synonym Protein Names
Ig lambda chain V-I region BL2
UniProt Gene Name
IGLV1-51  [Similar Products]
UniProt Comments for IGL
V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Product References and Citations for anti-IGL antibody
Campbell JP et. al. J Immunol Methods. 2013; 391(1-2):1-13.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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