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anti-LITAF antibody :: Mouse anti-Human LITAF Monoclonal Antibody

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Catalog # MBS200149
Unit / Price
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  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Western Blot (WB)
Product Name

LITAF, Monoclonal Antibody

Full Product Name

LITAF antibody

Product Synonym Names
lipopolysaccharide-induced TNF factor; PIG7; SIMPLE; FLJ38636; TP53I7
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
AF010312 mRNA
3D Structure
ModBase 3D Structure for Q99732
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT1F9
Host
Mouse
Species Reactivity
Human
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human LITAF (1-161aa) purified from E. coli
Clone Note
Anti-human LITAF mAb, clone AT5C10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human LITAF protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-LITAF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-LITAF antibody
Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) mediates the expression of inflammatory cytokines such as TNF-alpha in Lipopolysaccharide-induced processes. LITAF binds to STAT6B, a member of the STAT6 family forming a complex on the TNF-alpha promoter that modulates TNF activity. High levels of expression of LITAF mRNA have been observed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen.
Product Categories/Family for anti-LITAF antibody
Applications Tested/Suitable for anti-LITAF antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-LITAF antibody
WB: 1:2000 ~ 3000.
Recommended starting dilution is 1:2000.

Western Blot (WB) of anti-LITAF antibody
The recombinant human LITAF protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LITAF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant protein Lane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate Lane 4.: A431 cell lysate Lane 5.: SW480 cell lysate
anti-LITAF antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for LITAF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,824 Da
NCBI Official Full Name
lipopolysaccharide-induced tumor necrosis factor-alpha factor isoform 1
NCBI Official Synonym Full Names
lipopolysaccharide induced TNF factor
NCBI Official Symbol
LITAF  [Similar Products]
NCBI Official Synonym Symbols
PIG7; SIMPLE; TP53I7
  [Similar Products]
NCBI Protein Information
lipopolysaccharide-induced tumor necrosis factor-alpha factor
UniProt Protein Name
Lipopolysaccharide-induced tumor necrosis factor-alpha factor
UniProt Synonym Protein Names
Small integral membrane protein of lysosome/late endosome
UniProt Gene Name
LITAF  [Similar Products]
UniProt Synonym Gene Names
; LPS-induced TNF-alpha factor  [Similar Products]
NCBI Summary for LITAF
Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppressor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2014]
UniProt Comments for LITAF
Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation (PubMed:23166352). Plays a role in targeting endocytosed EGFR and ERGG3 for lysosomal degradation, and thereby helps downregulate downstream signaling cascades (PubMed:23166352). Helps recruit the ESCRT complex components TSG101, HGS and STAM to cytoplasmic membranes (PubMed:23166352). Probably plays a role in regulating protein degradation via its interaction with NEDD4 (PubMed:15776429). May also contribute to the regulation of gene expression in the nucleus (PubMed:10200294, PubMed:15793005). Binds DNA (in vitro) and may play a synergistic role with STAT6 in the nucleus in regulating the expression of various cytokines (PubMed:15793005). May regulate the expression of numerous cytokines, such as TNF, CCL2, CCL5, CXCL1, IL1A and IL10 (PubMed:10200294, PubMed:15793005).
Product References and Citations for anti-LITAF antibody
Atucchi A, et al., (2006) Inflamm Bowel Dis 13 (1):120-121; Tang X, et al., (2005) Proc Natl Acad Sci USA 102 (14):5132-5137; Tang X, et al., (2003) Proc Natl Acad Sci USA 100 (7):4096-4101

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Pathways associated with anti-LITAF antibody
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