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anti-MME antibody :: Mouse anti-Human MME / CD10 Monoclonal Antibody

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Catalog # MBS245033
Unit / Price
  0.05 mL  /  $530 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

MME / CD10, Monoclonal Antibody

Also Known As

Mouse Monoclonal (IgG2b) to Human MME / CD10

Product Synonym Names
Anti-MME / CD10 Antibody IHC-plus; MME; Atriopeptidase; CALLA; CD10; EPN; Membrane metallo-endopeptidase; NEP; Neprilysin; Neutral endopeptidase; Neutral endopeptidase 24.11; CD10 antigen; Enkephalinase; Neprylisin; SFE; Skin fibroblast elastase; Human MME; CD10
Product Gene Name
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
750
OMIM
120520
3D Structure
ModBase 3D Structure for P08473
Clonality
Monoclonal
Isotype
IgG2b
Host
Mouse
Species Reactivity
Human
Specificity
Ni-NTA purified recombinant human CD-10 expressed in E Coli strain BL21 (DE3)
Purity/Purification
Protein G Purified
Form/Format
PBS, pH 7.4, 0.03% sodium azide.
Concentration
1 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Recombinant human MME / CD10.
Immunogen Type
Recombinant protein
Immunogen
MME / CD10 antibody was raised against recombinant human MME / CD10.
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
Other Notes
Small volumes of anti-MME antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MME antibody
MME, also known as Neprilysin or CALLA for common acute lymphocytic leukemia antigen, is a Metallopeptidase M13 type protease. MME functions as a peptidase and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. The protein is found on leukemic cells of pre-B phenotype and serves as a cell-surface marker in the diagnosis of human acute lymphocytic leukemia (ALL).
Applications Tested/Suitable for anti-MME antibody
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), ELISA (EIA)
Application Notes for anti-MME antibody
ELISA (1:10000), IHC-Fr (1:200 - 1:400), IHC-P (1:100 - 1:200)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-MME antibody
Anti-CD10 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
anti-MME antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for MME. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
85,514 Da
NCBI Official Full Name
neprilysin
NCBI Official Synonym Full Names
membrane metallo-endopeptidase
NCBI Official Symbol
NCBI Official Synonym Symbols
NEP; SFE; CD10; CALLA
  [Similar Products]
NCBI Protein Information
neprilysin; enkephalinase; atriopeptidase; neprilysin-390; neprilysin-411; skin fibroblast elastase; neutral endopeptidase 24.11; membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; common acute lymphocytic leukemia antigen; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10)
UniProt Protein Name
Neprilysin
UniProt Synonym Protein Names
Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD_antigen: CD10
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
EPN; CALLA; NEP; Neutral endopeptidase; SFE  [Similar Products]
UniProt Entry Name
NEP_HUMAN
NCBI Summary for MME
This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq, Jul 2008]
UniProt Comments for MME
CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family.

Protein type: EC 3.4.24.11; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q25.2

Cellular Component: synaptic vesicle; focal adhesion; axon; integral to plasma membrane; cytoplasm; dendrite; plasma membrane; integral to membrane; synapse; brush border

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity; exopeptidase activity; peptide binding

Biological Process: peptide metabolic process; cellular protein metabolic process; beta-amyloid metabolic process; creatinine metabolic process; angiotensin maturation; kidney development; proteolysis; sensory perception of pain

Disease: Membranous Nephropathy, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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