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anti-MUC1 antibody :: anti-Human MUC1 (CD227) Monoclonal Antibody

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Catalog # MBS570208
Unit / Price
  0.1 mg  /  $410 +1 FREE 8GB USB
anti-MUC1 antibody
Product Name

MUC1 (CD227), Monoclonal Antibody

Popular Item
Also Known As

Mouse anti MUC1 (CD227)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (155158300..155162768, complement). Location: 1q21
OMIM
113720
3D Structure
ModBase 3D Structure for P15941
Clonality
Monoclonal
Isotype
IgG1
Clone Number
175C5
Host
Mouse (Balb/c)
Species Reactivity
Human
Specificity
175C5 shows a high preference for breast carcinomas relative to normal breast epithelium. The antibdy reacts with a carcinoma-associated antigen in both adenocarcinomas and squamous cell carcinomas of different origins.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Preparation and Storage
Store at 4°C, or in small aliquots at -20°C.
Other Notes
Small volumes of anti-MUC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MUC1 antibody
Mucin 1, cell surface associated (MUC-1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in Humans. MUC-1 is a glycoprotein with extensive O-linked glycosylation of its extracellular domain. Mucins line the apical surface of epithelial cells in the lungs, stomach, intestines, eyes and several other organs. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with colon, breast, ovarian, lung and pancreatic cancers.
Applications Tested/Suitable for anti-MUC1 antibody
Electron microscopy, ELISA, Flow cytometry,Immunoblotting,Immunofluorescence,Immunohistochemistry (frozen),Immunoprecipitation
Application Notes for anti-MUC1 antibody
140 H2 is useful for ELISA, immunofluorescence, flow cytometry, immunoprecipitation, western blot, electron microscopy and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration.
NCBI/Uniprot data below describe general gene information for MUC1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
122,102 Da[Similar Products]
NCBI Official Full Name
mucin-1 isoform 3
NCBI Official Synonym Full Names
mucin 1, cell surface associated
NCBI Official Symbol
NCBI Official Synonym Symbols
EMA; PEM; PUM; KL-6; MAM6; PEMT; CD227; H23AG; MCKD1; MUC-1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
  [Similar Products]
NCBI Protein Information
mucin-1; episialin; DF3 antigen; H23 antigen; cancer antigen 15-3; krebs von den Lungen-6; mucin 1, transmembrane; tumor-associated mucin; carcinoma-associated mucin; polymorphic epithelial mucin; peanut-reactive urinary mucin; tumor associated epithelial mucin; breast carcinoma-associated antigen DF3; tumor-associated epithelial membrane antigen
UniProt Protein Name
Mucin-1
UniProt Synonym Protein Names
Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD_antigen: CD227Cleaved into the following 2 chains:Mucin-1 subunit alpha; MUC1-NT; MUC1-alpha; Mucin-1 subunit beta; MUC1-betaAlternative name(s):MUC1-CT
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta  [Similar Products]
UniProt Entry Name
MUC1_HUMAN
NCBI Summary for MUC1
This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]
UniProt Comments for MUC1
MUC1: a large cell surface glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. Plays a role in adhesion and cell-cell interactions, metastasis and signaling. May provide a protective layer on epithelial surfaces. Direct or indirect interaction with actin cytoskeleton. Its cytoplasmic tail (MUC1CT) is involved in several signaling pathways, including those involving Ras, beta-catenin, p120 catenin, p53 and estrogen receptor alpha. MUC1CT also forms complexes with transcription factors, and then translocates to the nucleus by an unknown mechanism, where it is believed to influence the transcription of their target genes. MUC1CT has also been proposed to localize to mitochondrial membranes under conditions of genotoxic stress, where it attenuates the apoptotic pathway in response and confers resistance to apoptosis-inducing drugs. Aberrantly glycosylated forms expressed in human epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Nine alternatively spliced isoforms have been described. Isoforms 5 and 9 are secreted. Isoform 7, expressed only in tumor cells, is a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling. Can bind to GRB2 adapter protein.

Protein type: Nuclear receptor co-regulator; Membrane protein, integral; Actin-binding; Tumor suppressor; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; cell surface; integral to plasma membrane; Golgi lumen; apical plasma membrane; nuclear chromatin; cytoplasm; vesicle

Molecular Function: protein binding; p53 binding; transcription cofactor activity

Biological Process: protein amino acid O-linked glycosylation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; regulation of transcription from RNA polymerase II promoter in response to stress; cellular protein metabolic process; O-glycan processing; post-translational protein modification; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest

Disease: Medullary Cystic Kidney Disease 1
Product References and Citations for anti-MUC1 antibody
J. Hilkens, J. Wesseling, H. L. Vos, J. STorm, M. Boer, S. W. van der Valk and M. C. E. Maas, Episialin/CA15-3: its structure and involvement in breast cancer progression, Ned Tijdschr Klin Chem 1995; 20: 293-298

Precautions
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Disclaimer
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