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anti-Mbp antibody :: Mouse anti-Human Myelin Basic Protein Monoclonal Antibody

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Catalog # MBS609747
Unit / Price
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  0.05 mg  /  $455 +1 FREE 8GB USB
  0.1 mg  /  $745 +1 FREE 8GB USB
anti-Mbp antibody
Product Name

Myelin Basic Protein (Mbp), Monoclonal Antibody

Full Product Name

Myelin Basic Protein, phosphorylated (MBP)

Product Synonym Names
Anti -Myelin Basic Protein, phosphorylated (MBP)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 18; NC_005117.2 (78943608..79057329). Location: 18q
3D Structure
ModBase 3D Structure for P02688
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
0.T.90
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes human phosphorylated MBP and most common vertebrate species.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide before the addition of glycerol to 30%.
Immunogen
Synthetic peptide corresponding to the human myelin basic protein sequence phosphorylated at Thr98 (tuberculin coupled).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-Mbp antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Mbp antibody
Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles. MBP serves as a marker for oligodendrocytes and Schwann cells. MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis.
Product Categories/Family for anti-Mbp antibody
Applications Tested/Suitable for anti-Mbp antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-Mbp antibody
Suitable for use in Immunoblot, Protein Kinase Assay and Immunocytochemistry.
Dilution: Immunoblot: 0.5-2ug/ml. Strongly detects phosphorylated MBP. Note: Some preparations of MBP contain basal levels of phosphorylated MBP, which are detected by the M9758-05.
Protein Kinase Assay: Phosphorylated MBP was detected in a direct kinase assay using non-radioactive MAP Kinase Assay Kit, BioAssay (M2353) and usign MAP Kinase Immunoprecipitation Kinase Assay Kit, BioAssay (M2353-50).
Immunocytochemistry: Reported by an indepeend lab (3).
NCBI/Uniprot data below describe general gene information for Mbp. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,502 Da[Similar Products]
NCBI Official Full Name
myelin basic protein isoform 4
NCBI Official Synonym Full Names
myelin basic protein
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
Golli-Mbp; myelin basic protein; MBP S; myelin basic protein S; myelin basic protein Golli-mbp; Golli-Mbp; myelin basic protein; myelin basic protein S
UniProt Protein Name
Myelin basic protein S
Protein Family
UniProt Gene Name
UniProt Entry Name
MBP_RAT
NCBI Summary for Mbp
The protein encoded by the classic Mbp gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, Mbp-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long Mbp gene (otherwise called "Golli-Mbp") that contains 3 additional exons located upstream of the classic Mbp exons. Alternative splicing from the Golli and the Mbp transcription start sites gives rise to 2 sets of Mbp-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-Mbp, spliced in-frame to 1 or more Mbp exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to Mbp aa sequence. The second family of transcripts contain only Mbp exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the Mbp transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Mutation of the Mbp gene is associated with the 'shiverer' and 'myelin deficient' phenotypes in mouse. [provided by RefSeq]
UniProt Comments for Mbp
MBP: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. Homodimer. Isoform 3 exists as a homodimer. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Belongs to the myelin basic protein family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Cellular Component: compact myelin; internode region of axon; cell projection; cell soma; plasma membrane; myelin sheath

Molecular Function: protein binding; structural constituent of myelin sheath; protease binding

Biological Process: myelination; response to mercury ion; substantia nigra development; response to toxin; negative regulation of axonogenesis; response to progesterone stimulus; aging
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-Mbp antibody
 Products by Pathway  Pathway Diagram
 MAPK Cascade Pathway antibodies  MAPK Cascade Pathway Diagram
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