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anti-MYL4 antibody :: Mouse anti-Human MYL4 Monoclonal Antibody

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Catalog # MBS200272
Unit / Price
  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Western Blot (WB)
Product Name

MYL4, Monoclonal Antibody

Popular Item
Also Known As

MYL4 antibody

Product Synonym Names
Myosin light chain 4; Myosin; light chain 4; ALC1; AMLC; GT1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
197
OMIM
M36172 mRNA
3D Structure
ModBase 3D Structure for P12829
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
AT4E8
Host
Mouse
Species Reactivity
Human
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human MYL4 (1-197aa) purified from E. coli
Clone Note
Anti-human MYL4 mAb, clone AT4E8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MYL4 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-MYL4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MYL4 antibody
Myosin light chain 4 is a protein that in humans is encoded by the MYL4 gene. Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Product Categories/Family for anti-MYL4 antibody
Applications Tested/Suitable for anti-MYL4 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-MYL4 antibody
Recommended starting dilution is 1:1000.

Western Blot (WB) of anti-MYL4 antibody
The cell lysate of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: K562 cell lysate Lane 3.: MCF7 cell lysate Lane 4.: Jurkat cell lysate Lane 5.: 293T cell lysate Lane 6.: LnCap cell lysate
anti-MYL4 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for MYL4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,565 Da
NCBI Official Full Name
myosin light chain 4
NCBI Official Synonym Full Names
myosin light chain 4
NCBI Official Symbol
NCBI Official Synonym Symbols
GT1; ALC1; AMLC; PRO1957
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NCBI Protein Information
myosin light chain 4
UniProt Protein Name
Myosin light chain 4
UniProt Synonym Protein Names
Myosin light chain 1, embryonic muscle/atrial isoform; Myosin light chain alkali GT-1 isoform
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NCBI Summary for MYL4
Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for MYL4
Regulatory light chain of myosin. Does not bind calcium.
Product References and Citations for anti-MYL4 antibody
Kurabayashi, M., et al. (1998) J Biol Chem 263 (27): 13930-6; Rotter, M., et al. (1991) Nucleic Acids Res 19 (7): 1497-504;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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