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anti-NPHS1 antibody :: Rabbit anti-Human Nephrin Monoclonal Antibody

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Catalog # MBS684285
Unit / Price
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  0.1 mL  /  $210 +1 FREE 8GB USB
  100 Tests  /  $915 +1 FREE 8GB USB
Flow Cytometry (FC/FACS)
Product Name

Nephrin (NPHS1), Monoclonal Antibody

Popular Item
Full Product Name

Anti-Nephrin FITC

Product Synonym Names
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clone Number
Species Reactivity
Aqueous buffer solution containing 10mg/ml of BSA and 0.05% sodium azide.
0.2 mg/mL clonal IgGs (lot specific)
The antibody was prepared by in vitro cloning technology, and the monospecific product was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Important Notes
This antibody detects intracellular antigen (cells of interest must be permeabilized).
Optimal dilution should be determined by the end user.
Use 1×106 cells in a 100 ul experimental sample (a test).
This product is for in vitro use only, research or diagnostic applications.
Product contains sodium azide.
Preparation and Storage
Store undiluted at 4°C, protect from prolonged exposure to light. Do not freeze.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Other Notes
Small volumes of anti-NPHS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NPHS1 antibody
FITC Rabbit Anti-Human Nephrin
Applications Tested/Suitable for anti-NPHS1 antibody
This antibody detects intracellular antigen( cells of interest must be permeabilized).

Optimal dilutions should be determined by the end user.
Application Notes for anti-NPHS1 antibody
Use 1 x 10 6 cells in a 100 uL experimental sample (a test).

Flow Cytometry (FC/FACS) of anti-NPHS1 antibody
Profile of nephrin expression in human Hek293 cell line analyzed.

Cells were fixed, permeabilized and stained with anti-Nephrin FITC (green, used 10 ul per test) or with an isotype control (black).
anti-NPHS1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for NPHS1. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
NCBI Official Synonym Full Names
NPHS1 nephrin
NCBI Official Symbol
NPHS1  [Similar Products]
NCBI Official Synonym Symbols
CNF; NPHN; nephrin
  [Similar Products]
NCBI Protein Information
UniProt Protein Name
UniProt Synonym Protein Names
Renal glomerulus-specific cell adhesion receptor
Protein Family
UniProt Gene Name
NPHS1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
NCBI Summary for NPHS1
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]
UniProt Comments for NPHS1
NPHS1: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1. Interacts with NPHS2. Specifically expressed in podocytes of kidney glomeruli. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cell projection; integral to plasma membrane; intracellular; plasma membrane

Molecular Function: myosin binding; protein binding

Biological Process: cell adhesion; excretion; glomerular basement membrane development; JNK cascade; myoblast fusion; positive regulation of actin filament polymerization; skeletal muscle development

Disease: Nephrotic Syndrome, Type 1
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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