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anti-NKX2.2 antibody :: Rabbit NKX2.2 Monoclonal Antibody

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Catalog # MBS4380141
Unit / Price
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  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
Product Name

NKX2.2, Monoclonal Antibody

Popular Item
Full Product Name

NKX2.2 (Neuroendocrine & Ewing's Sarcoma Marker)

Product Synonym Names
Homeobox protein NK-2 homolog B; NK2 transcription factor like protein B; NK2 transcription factor related locus 2; NKX22; Nkx2b; tinman
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O95096
Clonality
Monoclonal
Isotype
IgG
Clone Number
NX2/2198R
Host
Rabbit
Species Reactivity
Human. Others not known.
Specificity
Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Immunogen
Human recombinant NKX2.2 protein fragment (around aa1-119)
Cellular Localization
Nuclear
Hu-chromosome Location
20p11.22
Positive Control
Pancreas or Ewings sarcoma
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-NKX2.2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-NKX2.2 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-NKX2.2 antibody
FC/FACS: 0.5-1ug/million cells
IF: 1-2ug/ml
IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-NKX2.2 antibody
Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with NKX2.2 Rabbit Recombinant Monoclonal Antibody (NX2/2198R).
anti-NKX2.2 antibody Immunohistochemistry (IHC) (IHC) image
SDS-PAGE Analysis of Purified NKX2.2 Rabbit Recombinant Monoclonal Antibody (NX2/2198R).
anti-NKX2.2 antibody SDS-Page image
NCBI/Uniprot data below describe general gene information for NKX2.2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
40-50kDa
NCBI Official Full Name
homeobox protein Nkx-2.2
NCBI Official Synonym Full Names
NK2 homeobox 2
NCBI Official Symbol
NKX2-2  [Similar Products]
NCBI Official Synonym Symbols
NKX2B; NKX2.2
  [Similar Products]
NCBI Protein Information
homeobox protein Nkx-2.2
UniProt Protein Name
Homeobox protein Nkx-2.2
UniProt Synonym Protein Names
Homeobox protein NK-2 homolog B
UniProt Gene Name
NKX2-2  [Similar Products]
UniProt Synonym Gene Names
NKX2.2; NKX2B  [Similar Products]
NCBI Summary for NKX2.2
The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. [provided by RefSeq, Jul 2008]
UniProt Comments for NKX2.2
Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter ().
Product References and Citations for anti-NKX2.2 antibody
Yoshida A et. al. Am J Surg Pathol. 2012; 36(7):993-9. Wang, Y.C. et al. (2009)Endocr Relat Cancer16, 267-79.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Organs/Tissues associated with anti-NKX2.2 antibody
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