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anti-OPN antibody :: Mouse anti-Human Osteopontin Monoclonal Antibody

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Catalog # MBS1488153
Unit / Price
  0.05 mg  /  $185 +1 FREE 8GB USB
  0.1 mg  /  $265 +1 FREE 8GB USB
Product Name

Osteopontin (OPN), Monoclonal Antibody

Popular Item
Also Known As

Mouse anti-Human Osteopontin monoclonal Antibody

Product Synonym Names
Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin; BNSP; PSEC0156
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS1488153 Technical Datasheet
Immunogen Sequence Length
300
OMIM
166490
3D Structure
ModBase 3D Structure for P10451
Clonality
Monoclonal
Isotype
IgG2a
Host
Mouse
Species Reactivity
Human
Specificity
Specific for human Osteopontin denatured and native forms
Purity/Purification
>95%, Protein G Purified
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant Human Osteopontin
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-21742 / sc-73631
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-OPN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-OPN antibody
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity
Applications Tested/Suitable for anti-OPN antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Immunohistochemistry (IHC) of anti-OPN antibody
Immunohistochemical of paraffin-embedded human colon cancer using MBS1488153 at dilution of 1:200
anti-OPN antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-OPN antibody
Immunohistochemical of paraffin-embedded human Kidney tissue using MBS1488153 at dilution of 1:200
anti-OPN antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-OPN antibody
All Lanes Mouse anti human OPN monoclonal antibody at at 2ug/ml
Lane 1:mouse kidney tissue lysate
Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 34.5 kDa
Observed band size: 33,36,34kDa
Additional bands at: 50 kDa.
anti-OPN antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for OPN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
33,843 Da
NCBI Official Full Name
osteopontin isoform OPN-b
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
NCBI Official Synonym Symbols
OPN; BNSP; BSPI; ETA-1
  [Similar Products]
NCBI Protein Information
osteopontin
UniProt Protein Name
Osteopontin
UniProt Synonym Protein Names
Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
BNSP; OPN; SPP-1  [Similar Products]
UniProt Entry Name
OSTP_HUMAN
NCBI Summary for OPN
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Comments for OPN
osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: apical part of cell; cell projection; extracellular region; extracellular space; perinuclear region of cytoplasm

Molecular Function: cytokine activity; extracellular matrix binding; protein binding

Biological Process: biomineral formation; cell adhesion; decidualization; embryo implantation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; inflammatory response; negative regulation of collateral sprouting of intact axon in response to injury; neutrophil chemotaxis; osteoblast differentiation; positive regulation of bone resorption; response to steroid hormone stimulus; response to vitamin D
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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