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anti-F2RL1 antibody :: Mouse anti-Human PAR2 Monoclonal Antibody

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Catalog # MBS601417
Unit / Price
  100 Tests  /  $685 +1 FREE 8GB USB
anti-F2RL1 antibody
Product Name

PAR2 (F2RL1), Monoclonal Antibody

Popular Item
Full Product Name

PAR2 (Proteinase Activated Receptor 2) (PE)

Product Synonym Names
Anti -PAR2 (Proteinase Activated Receptor 2) (PE)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 5; NC_000005.9 (76114833..76131140). Location: 5q13
OMIM
600933
3D Structure
ModBase 3D Structure for P55085
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
7H40
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes human PAR2.
Purity/Purification
Purified
Purified
Form/Format
Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).
Concentration
0.05 mg/ml (lot specific)
Immunogen
NS0 mouse myeloma cell line transfected with human PAR2, Accession # P55085
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C. Stable for 12 months at 4 degree C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Other Notes
Small volumes of anti-F2RL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-F2RL1 antibody
Human protease-activated receptor 2 (PAR2) is one of 4 members in a unique subclass of the Class A G protein- coupled receptor superfamily. Receptor activation involves the proteolytic uncovering of a cryptic N-terminus receptor sequence that forms a tethered ligand and binds to the receptors within the extracellular loop 2. This activates the receptors to initiate multiple signaling cascades. In addition, short peptides (5-6 amino acids) synthesized based on the proteolytically revealed motif can activate the receptors without the cleavage by enzymes. PAR2 is activated by trypsin and trypsin-like enzymes, and the cleavage site has been identified at residue Arg 36. PAR2 plays an important role in the regulation of cardiovascular, gastrointestinal, and skin function. PAR2 is expressed in human vascular tissues, pancreas, kidney, colon, liver, small intestine, and some tumor cell lines (1, 2).
Product Categories/Family for anti-F2RL1 antibody
Applications Tested/Suitable for anti-F2RL1 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-F2RL1 antibody
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: Use 10ul to label 1x10e5 cells or 50ul of packed whole blood.
NCBI/Uniprot data below describe general gene information for F2RL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,126 Da[Similar Products]
NCBI Official Full Name
proteinase-activated receptor 2
NCBI Official Synonym Full Names
coagulation factor II (thrombin) receptor-like 1
NCBI Official Symbol
F2RL1  [Similar Products]
NCBI Official Synonym Symbols
PAR2; GPR11
  [Similar Products]
NCBI Protein Information
proteinase-activated receptor 2; PAR-2; OTTHUMP00000128315; thrombin receptor-like 1; G protein-coupled receptor-11; G-protein coupled receptor 11; protease-activated receptor 2; proteinase-activated receptor-2; coagulation factor II receptor-like 1
UniProt Protein Name
Proteinase-activated receptor 2
UniProt Synonym Protein Names
Coagulation factor II receptor-like 1; G-protein coupled receptor 11; Thrombin receptor-like 1
UniProt Gene Name
F2RL1  [Similar Products]
UniProt Synonym Gene Names
GPR11; PAR2  [Similar Products]
UniProt Entry Name
PAR2_HUMAN
NCBI Summary for F2RL1
Coagulation factor II (thrombin) receptor-like 1 (F2RL1) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL1 is also a member of the protease-activated receptor family. It is activated by trypsin, but not by thrombin. It is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. The F2RL1 gene contains two exons and is widely expressed in human tissues. The predicted protein sequence is 83% identical to the mouse receptor sequence. [provided by RefSeq]
UniProt Comments for F2RL1
F2RL1: a G-protein coupled receptor for trypsin and trypsin-like enzymes. Acts as a sensor for proteolytic enzymes generated during infection. Modulates pro-inflammatory responses, and innate and adaptive immunity. It is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. Activates several signaling molecules including phospholipase C (PLC), mitogen-activated protein kinase (MAPK), IKK/NFkB and Rho. Elevates intracellular calcium. Can also be transactivated by cleaved PAR1. Can signal synergistically with TLR4 and probably TLR2 in inflammatory responses and modulates TLR3 signaling. Has a protective role in establishing the endothelial barrier; the activity involves coagulation factor X. Proposed to have a bronchoprotective role in airway epithelium, but also shown to compromise the airway epithelial barrier by interrupting E-cadherin adhesion. Involved in the regulation of vascular tone; activation results in hypotension presumably mediated by vasodilation. Associates with a subset of G proteins alpha subunits such as G alpha-q, G alpha-11, G alpha-14, G alpha- 12 and G alpha-13, but probably not with G(o) alpha, G(i) subunit alpha-1 and G(i) subunit alpha-2. However, may signal through G(i) subunit alpha. Believed to be a class B receptor that internalizes as a complex with arrestin and traffic with it to endosomal vesicles, presumably as desensitized receptor, for extended periods of time. Mediates inhibition of TNF-alpha stimulated JNK phosphorylation via coupling to G alpha-q/11; the function involves dissociation of RIPK1 and TRADD from TNFR1. Involved in cellular migration. Involved in cytoskeletal rearrangement and chemotaxis through beta-arrestin-promoted scaffolds; the function is independent of G alpha-q/11 and involves promotion of cofilin dephosphoryltaion and actin filament severing. Induces redistribution of COPS5 from the plasma membrane to the cytosol and activation of the JNK cascade is mediated by COPS5. Involved in the recruitment of leukocytes to the sites of inflammation and is the major PAR receptor capable of modulating eosinophil function such as proinflammatory cytokine secretion, superoxide production and degranulation. During inflammation promotes dendritic cell maturation, trafficking to the lymph nodes and subsequent T-cell activation. Involved in antimicrobial response of innate immune cells; activation enhances phagocytosis of Gram- positive and killing of Gram-negative bacteria. Acts synergistically with interferon-gamma in enhancing antiviral responses. Implicated in a number of acute and chronic inflammatory diseases such as of the joints, lungs, brain, gastrointestinal tract, periodontium, skin, and vascular systems, and in autoimmune disorders. Widely expressed in tissues with especially high levels in pancreas, liver, kidney, small intestine, and colon. Moderate expression is detected in many organs, but none in brain or skeletal muscle. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: Golgi apparatus; integral to plasma membrane; early endosome; plasma membrane; pseudopodium

Molecular Function: G-protein coupled receptor activity; protein binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; receptor activity; receptor binding; thrombin receptor activity

Biological Process: negative regulation of JNK cascade; positive regulation of positive chemotaxis; positive regulation of cytokine secretion during immune response; positive regulation of JNK cascade; positive regulation of leukocyte chemotaxis; positive regulation of vasodilation; regulation of JNK cascade; negative regulation of toll-like receptor 3 signaling pathway; T cell activation during immune response; elevation of cytosolic calcium ion concentration; positive regulation of glomerular filtration; positive regulation of superoxide release; interleukin-1 beta secretion; inflammatory response; regulation of I-kappaB kinase/NF-kappaB cascade; defense response to virus; positive regulation of toll-like receptor 3 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; neutrophil activation; positive regulation of actin filament depolymerization; positive regulation of chemotaxis; positive regulation of eosinophil degranulation; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of Rho protein signal transduction; positive regulation of toll-like receptor 2 signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; positive regulation of pseudopodium formation; regulation of blood coagulation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; positive regulation of phagocytosis, engulfment; leukocyte migration; positive regulation of cell migration
Precautions
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Disclaimer
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