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anti-PAX-8 antibody :: Mouse anti-Human PAX-8 Monoclonal Antibody

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Catalog # MBS346026
Unit / Price
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  0.01 mL  /  $170 +1 FREE 8GB USB
  0.1 mL  /  $380 +1 FREE 8GB USB
  0.2 mL  /  $540 +1 FREE 8GB USB
  0.5 mL  /  $1,015 +1 FREE 8GB USB
  1 mL  /  $1,540 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

PAX-8, Monoclonal Antibody

Full Product Name

PAX-8 Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q99573
Clonality
Monoclonal
Isotype
IgG
Clone Number
IHC008
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid
Buffer
Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3
IHC Positive Control
Ovarian, Thyroid, and Renal Cell Carcinomas
IHC Suggested Protocol
1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block – Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.)
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
Other Notes
Small volumes of anti-PAX-8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PAX-8 antibody
PAX-8 is a transcription factor in the paired box (PAX) family, whose mainly functions as important regulators in early development, especially in thyroid follicular cells and thyroid specific genes. Mutations in PAX-8 could lead to thyroid follicular carcinoams, atypical thyroid adenomas, and thyroid dysgenesis. Studies have associated PAX-8 expression with renal carcinoma, nephroblastoma, and seminoma, and have indicated PAX-8 as a useful marker for renal epithelial tumors, ovarian cancer, and for differential diagnoses in lung and neck tumors.
Applications Tested/Suitable for anti-PAX-8 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-PAX-8 antibody
Dilution Range: 1:100 - 1:200

Immunohistochemistry (IHC) of anti-PAX-8 antibody
IHC (paraffin) of human thyroid gland using PAX-8 Antibody
anti-PAX-8 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PAX-8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
6,524 Da
NCBI Official Full Name
Pax-8, partial
NCBI Official Synonym Full Names
paired box 8
NCBI Official Symbol
NCBI Protein Information
paired box protein Pax-8
UniProt Protein Name
Pax-8 protein
UniProt Synonym Protein Names
Pax-8 protein
UniProt Gene Name
Pax-8  [Similar Products]
NCBI Summary for PAX-8
This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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