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anti-PBK antibody :: Mouse anti-Human PBK Monoclonal Antibody

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Catalog # MBS9602541
Unit / Price
  0.05 mL  /  $235 +1 FREE 8GB USB
  0.1 mL  /  $295 +1 FREE 8GB USB
  0.2 mL  /  $370 +1 FREE 8GB USB
Western Blot (WB)
Product Name

PBK, Monoclonal Antibody

Popular Item
Also Known As

PBK Antibody

Product Synonym Names
Cancer/testis antigen 84; CT84; Epididymis luminal protein 164; FLJ14385; HEL164; Lymphokine activated killer T cell originated protein kinase; Lymphokine-activated killer T-cell-originated protein kinase; MAPKK like protein kinase; MAPKK-like protein kinase; Nori 3; Nori-3; Nori3; PBK; PDZ binding kinase; PDZ-binding kinase; Serine/threonine protein kinase; Spermatogenesis related protein kinase; Spermatogenesis-related protein kinase; SPK; T LAK cell originated protein kinase; T-LAK cell-originated protein kinase; TOPK; TOPK_HUMAN
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
333
3D Structure
ModBase 3D Structure for Q96KB5
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human
Specificity
PBK antibody detects endogenous levels of total PBK
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
Purified recombinant fragment of human PBK expressed in E. Coli
Tissue Specificity
Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-PBK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PBK antibody
Function: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Subunit Structure: Interacts with DLG1 and TP53.
Post-translational Modifications: Phosphorylated; in a cell-cycle dependent manner at mitosis.
Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Applications Tested/Suitable for anti-PBK antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes for anti-PBK antibody
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
FC/FACS: 1:200-1:400

Western Blot (WB) of anti-PBK antibody
Figure 2: Western blot analysis using PBK mAb against human PBK (AA: 50-230) recombinant protein. (Expected MW is 45.8 kDa) 
anti-PBK antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for PBK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 36 kDa
Predicted: 37 kDa
NCBI Official Full Name
lymphokine-activated killer T-cell-originated protein kinase isoform 2
NCBI Official Synonym Full Names
PDZ binding kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
SPK; CT84; TOPK; HEL164; Nori-3
  [Similar Products]
NCBI Protein Information
lymphokine-activated killer T-cell-originated protein kinase
UniProt Protein Name
Lymphokine-activated killer T-cell-originated protein kinase
UniProt Synonym Protein Names
Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase
UniProt Gene Name
UniProt Synonym Gene Names
TOPK; CT84; SPK  [Similar Products]
NCBI Summary for PBK
This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Comments for PBK
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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