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anti-PDGFAA antibody :: Mouse anti-Human Platelet Derived Growth Factor AA (PDGFAA) Monoclonal Antibody

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Catalog # MBS2012496 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $90 +1 FREE 8GB USB
  0.02 mg  /  $100 +1 FREE 8GB USB
  0.05 mg  /  $130 +1 FREE 8GB USB
  0.1 mg  /  $160 +1 FREE 8GB USB
  0.2 mg  /  $200 +1 FREE 8GB USB
  1 mg  /  $430 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Platelet Derived Growth Factor AA (PDGFAA), Monoclonal Antibody

Popular Item
Also Known As

Monoclonal Antibody to Platelet Derived Growth Factor AA (PDGFAA)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-SIEE AVPAVCKTRT VIYEIPRSQV DPTSANFLIW PPCVEVKRCT GCCNTSSVKC QPSRVHHRSV KVAKVEYVRK KPKLKEVQVR LEEHLECACA
OMIM
X03795 mRNA
3D Structure
ModBase 3D Structure for P04085
Clonality
Monoclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Specificity
The antibody is a mouse monoclonal antibody raised against PDGFAA. It has been selected for its ability to recognize PDGFAA in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/ml (lot specific)
Immunogen
Recombinant PDGFAA (Ser87~Thr211) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043345
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-PDGFAA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PDGFAA antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-PDGFAA antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-PDGFAA antibody
Western Blot: Sample: Recombinant PDGFAA, Human.
anti-PDGFAA antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for PDGFAA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
22,253 Da
NCBI Official Full Name
platelet-derived growth factor subunit A isoform 1 preproprotein
NCBI Official Synonym Full Names
platelet derived growth factor subunit A
NCBI Official Symbol
PDGFA  [Similar Products]
NCBI Official Synonym Symbols
PDGF1; PDGF-A
  [Similar Products]
NCBI Protein Information
platelet-derived growth factor subunit A
UniProt Protein Name
Platelet-derived growth factor subunit A
UniProt Synonym Protein Names
PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide
UniProt Gene Name
PDGFA  [Similar Products]
UniProt Synonym Gene Names
PDGF1; PDGF subunit A  [Similar Products]
NCBI Summary for PDGFAA
This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
UniProt Comments for PDGFAA
PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: cell surface; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; Golgi membrane; microvillus

Molecular Function: collagen binding; growth factor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: actin cytoskeleton organization; alveolus development; angiogenesis; cell activation; cell projection assembly; cell-cell signaling; extracellular matrix organization; hair follicle development; MAPK cascade; negative chemotaxis; negative regulation of phosphatidylinositol biosynthetic process; organ morphogenesis; phosphatidylinositol phosphorylation; phosphoinositide-mediated signaling; platelet degranulation; platelet-derived growth factor receptor signaling pathway; positive regulation of cell division; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of fibroblast proliferation; positive regulation of GTPase activity; positive regulation of MAP kinase activity; positive regulation of MAPK cascade; positive regulation of mesenchymal cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation; positive regulation of protein kinase B signaling; regulation of actin cytoskeleton organization; regulation of peptidyl-tyrosine phosphorylation; regulation of phosphatidylinositol 3-kinase signaling; regulation of smooth muscle cell migration; response to wounding; skin development; wound healing
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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