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anti-POU2F1 antibody :: Mouse anti-Human POU2F1 Monoclonal Antibody

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Catalog # MBS120010
Unit / Price
  0.1 mg  /  $345 +1 FREE 8GB USB
Product Name

POU2F1, Monoclonal Antibody

Full Product Name

Mouse monoclonal antibody Anti-Human POU2F1

Product Synonym Names
POU domain, class 2, transcription factor 1; OCT1; OTF1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
164175
Clonality
Monoclonal
Isotype
IgG1
Clone Number
POU5I097
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Other Notes
Small volumes of anti-POU2F1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-POU2F1 antibody
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). [UniProtKB Function]
Applications Tested/Suitable for anti-POU2F1 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB)

Quality Control of anti-POU2F1 antibody
Western blot analysis of immunized recombinant protein, using anti-POU2F1 monoclonal antibody.
anti-POU2F1 antibody Quality Control image
Quality Control #2 of anti-POU2F1 antibody
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
anti-POU2F1 antibody Quality Control #2 image
Western Blot (WB) of anti-POU2F1 antibody
Detection of human POU2F1 by Western blot.

Samples: Whole cell lysate (50 ug) from HEK293 cells. [Lot No. POU5I097-1]

Predicted molecular weight: 76 kDa
anti-POU2F1 antibody Western Blot (WB) (WB) image
Immunocytochemistry (ICC) of anti-POU2F1 antibody
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-POU2F1 mAb. [Lot No. POU5I097-1]
anti-POU2F1 antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for POU2F1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
72,082 Da
NCBI Official Full Name
Homo sapiens POU class 2 homeobox 1, mRNA
NCBI Official Synonym Full Names
POU class 2 homeobox 1
NCBI Official Symbol
POU2F1  [Similar Products]
NCBI Official Synonym Symbols
OCT1; OTF1; oct-1B
  [Similar Products]
NCBI Protein Information
POU domain, class 2, transcription factor 1; NF-A1; OTF-1; Octamer-binding transcription factor-1; oct-1; octamer-binding protein 1; octamer-binding transcription factor 1
UniProt Protein Name
POU domain, class 2, transcription factor 1
UniProt Synonym Protein Names
NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1
UniProt Gene Name
POU2F1  [Similar Products]
UniProt Entry Name
PO2F1_HUMAN
NCBI Summary for POU2F1
The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]
UniProt Comments for POU2F1
Oct1: a ubiquitous transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. Phosphorylation apparently inhibits its interaction with DNA. Four splice variant isoforms have been described. Isoform 2 is lymphocyte specific.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: nucleoplasm; transcription factor complex; intracellular membrane-bound organelle; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase III promoter; positive regulation of transcription from RNA polymerase II promoter; gene expression; negative regulation of transcription, DNA-dependent; olfactory placode formation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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