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anti-PPP1CA antibody :: Mouse anti-Human PPP1CA Monoclonal Antibody

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Catalog # MBS200069
Unit / Price
  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Product Name

PPP1CA, Monoclonal Antibody

Also Known As

PPP1CA antibody

Product Synonym Names
serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 1; protein phosphatase 1 catalytic subunit alpha isozyme; PP-1A; PP1alpha; ppp1a; MGC15877; MGC1674;
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
330
OMIM
X70848 mRNA
3D Structure
ModBase 3D Structure for P62136
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
4G3
Host
Mouse
Species Reactivity
Human
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human PPP1CA (30-299aa) purified from E. coli
Clone Note
Anti-human PPP1CA mAb, clone 4G3, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPP1CA protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-PPP1CA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PPP1CA antibody
Protein phosphatase 1, catalytic subunit, alpha isoform, also known as PPP1CA, is one of the three catalytic subunits of protein phosphatase 1. The protein is highly conserved in eukaryotic cells and play a role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. It is participate in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis and apoptosis
Product Categories/Family for anti-PPP1CA antibody
Applications Tested/Suitable for anti-PPP1CA antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-PPP1CA antibody
WB: 1:500 ~ 2,000.
Recommended starting dilution is 1:1,000.

Western Blot (WB) of anti-PPP1CA antibody
Cell lysates of HepG2 (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1CA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
anti-PPP1CA antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-PPP1CA antibody
ICC/IF analysis of PPP1CA in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPP1CA antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
anti-PPP1CA antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for PPP1CA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
32,595 Da
NCBI Official Full Name
serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 1
NCBI Official Synonym Full Names
protein phosphatase 1 catalytic subunit alpha
NCBI Official Symbol
PPP1CA  [Similar Products]
NCBI Official Synonym Symbols
PP1A; PP-1A; PPP1A; PP1alpha
  [Similar Products]
NCBI Protein Information
serine/threonine-protein phosphatase PP1-alpha catalytic subunit
UniProt Protein Name
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
UniProt Gene Name
PPP1CA  [Similar Products]
UniProt Synonym Gene Names
PPP1A; PP-1A  [Similar Products]
NCBI Summary for PPP1CA
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for PPP1CA
Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Dephosphorylates CENPA (PubMed:25556658). Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy (PubMed:26083323).
Product References and Citations for anti-PPP1CA antibody
Cohen PT. (2002) J Cell Sci. 115 (2):241-56; Hsu LC, et al. (2006) Oncogene. 25 (40):5517-26

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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