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anti-F2 antibody :: Mouse anti-Human Prothrombin (Calcium dependant) Monoclonal Antibody

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Catalog # MBS520489
Unit / Price
  0.2 mg  /  $395 +1 FREE 8GB USB
anti-F2 antibody
Product Name

Prothrombin (Calcium dependant) (F2), Monoclonal Antibody

Popular Item
Also Known As

Anti-Human Prothrombin (Calcium dependant), Purified, (clone: Ca-Pro 20) (Mouse IgG1)

Product Synonym Names
Prothrombin (Calcium dependant), Purified, (clone: Ca-Pro 20) (Mouse IgG1); Calcium-Dependent Anti-Human Prothrombin (CaPro-20)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
64
Chromosome Location
Chromosome: 11; NC_000011.9 (46740743..46761056). Location: 11p11
OMIM
176930
3D Structure
ModBase 3D Structure for P00734
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
CaPro-20
Host
Mouse
Species Reactivity
Human
Specificity
This antibody is specific for Human Prothrombin (CaPro-20)
Form/Format
Purified
Concentration
1.0 mg/ml (lot specific)
Immunogen
Human Prothrombin (CaPro-20) purified from plasma.
Presentation
Purified IgG buffered in PBS and 0.02% NaN3. (Purified from ascitic fluid via Protein G Chromatography).
Preparation and Storage
Store at +4 degree C. For long term storage, aliquot and feeze unused portion at -20 degree C in volumes appropriate for single use. Avoid freeze thaw cycles.
Other Notes
Small volumes of anti-F2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-F2 antibody
Prothrombin is a 72 kDa blood clotting glycoprotein formed by and stored in the Liver. Prothrombin is present in the blood plasma and is converted to the key blood clotting agent thrombin, when blood vessels are damaged. Factor VII, another important haemostasis protein can be converted to VIIa by Factor IXa or thrombin.
Applications Tested/Suitable for anti-F2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-F2 antibody
This antibody is suitable for use in ELISA and Western Blot.
NCBI/Uniprot data below describe general gene information for F2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
70,037 Da[Similar Products]
NCBI Official Full Name
prothrombin
NCBI Official Synonym Full Names
coagulation factor II (thrombin)
NCBI Official Symbol
NCBI Official Synonym Symbols
PT; THPH1; RPRGL2
  [Similar Products]
NCBI Protein Information
prothrombin; serine protease; prothrombin B-chain; prepro-coagulation factor II
UniProt Protein Name
Prothrombin
UniProt Synonym Protein Names
Coagulation factor II
Protein Family
UniProt Gene Name
UniProt Entry Name
THRB_HUMAN
NCBI Summary for F2
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq, Jul 2008]
UniProt Comments for F2
Function: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Ref.13

Catalytic activity: Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.

Enzyme regulation: Inhibited by SERPINA5. Ref.11

Subunit structure: Heterodimer (named alpha-thrombin) of a light and a heavy chain; disulfide-linked. Forms a heterodimer with SERPINA5.

Subcellular location: Secreted › extracellular space.

Tissue specificity: Expressed by the liver and secreted in plasma.

Post-translational modification: The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). Ref.22

Involvement in disease: Factor II deficiency (FA2D) [MIM:613679]: A very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2 Ref.35 Ref.36 Ref.37 Ref.38 Ref.39 Ref.40 Ref.41 Ref.42 Ref.43 Ref.44 Ref.45Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.15Thrombophilia due to thrombin defect (THPH1) [MIM:188050]: A multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.Note: The disease is caused by mutations affecting the gene represented in this entry. A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis.Pregnancy loss, recurrent, 2 (RPRGL2) [MIM:614390]: A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.14

Pharmaceutical use: The peptide TP508 also known as Chrysalin (Orthologic) could be used to accelerate repair of both soft and hard tissues.

Miscellaneous: Prothrombin is activated on the surface of a phospholipid membrane that binds the amino end of prothrombin and factors Va and Xa in Ca-dependent interactions; factor Xa removes the activation peptide and cleaves the remaining part into light and heavy chains. The activation process starts slowly because factor V itself has to be activated by the initial, small amounts of thrombin.It is not known whether 1 or 2 smaller activation peptides, with additional cleavage after Arg-314, are released in natural blood clotting.Thrombin can itself cleave the N-terminal fragment (fragment 1) of the prothrombin, prior to its activation by factor Xa.The cleavage after Arg-198, observed in vitro, does not occur in plasma.

Sequence similarities: Belongs to the peptidase S1 family.Contains 1 Gla (gamma-carboxy-glutamate) domain.Contains 2 kringle domains.Contains 1 peptidase S1 domain.
Precautions
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Disclaimer
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