• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-RASSF1A antibody :: Mouse anti-Human RASSF1A Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS200009
Unit / Price
  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Product Name

RASSF1A, Monoclonal Antibody

Also Known As

RASSF1A antibody

Product Synonym Names
RASSF1 (Ras association (RalGDS/AF-6) domain family 1),123F2; RDA32; NORE2A; RASSF1A; REH3P21; Ras association (RalGDS/AF-6) domain family member 1;
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
AF061836 mRNA
3D Structure
ModBase 3D Structure for Q9NS23
IgG1, k
Clone Number
Species Reactivity
By protein-G affinity chromatography
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
1mg/ml (lot specific)
Antigen Species
Recombinant human RASSF1A (1-340aa) purified from E. coli
Clone Note
Anti-human RASSF1A mAb, clone 3F3, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant RASSF1A.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-RASSF1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RASSF1A antibody
The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability.
Product Categories/Family for anti-RASSF1A antibody
Applications Tested/Suitable for anti-RASSF1A antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Western Blot (WB) of anti-RASSF1A antibody
Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: RASSF1A Transfected 293T cell lysate
anti-RASSF1A antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-RASSF1A antibody
A431 cells were labeled with anti-RASSF1A (clone 3F3) mAb, and detection was using a biotinylated secondary antibody and Texas-red conj streptavidin. ICC/IF analysis of RASSF1A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RASSF1A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
anti-RASSF1A antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for RASSF1A. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
8,656 Da
NCBI Official Full Name
ras association domain-containing protein 1 isoform A
NCBI Official Synonym Full Names
Ras association domain family member 1
NCBI Official Symbol
RASSF1  [Similar Products]
NCBI Official Synonym Symbols
123F2; RDA32; NORE2A; RASSF1A; REH3P21
  [Similar Products]
NCBI Protein Information
ras association domain-containing protein 1
UniProt Protein Name
Ras association domain-containing protein 1
UniProt Gene Name
RASSF1  [Similar Products]
NCBI Summary for RASSF1A
This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, May 2011]
UniProt Comments for RASSF1A
Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
Product References and Citations for anti-RASSF1A antibody
Agathanggelou A, et al., (2005) Cancer Res. 65, 3497-3508; Lerman MI. et. al. (2000) Cancer Res. 60, 6116-6133; Strunnikova M. et al., (2005) Mol Cell Biol. 25, 3923-3933; Dammann R. et al., (2005) Histol Histopathol. 20, 645-663; Rossa W.k, et al., (2007) American Journal of pathology. 170, 941-950

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

Contact Us

Please fill out the form below and our representative will get back to you shortly.