• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-RB1 antibody :: Mouse anti-Human RB1 Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS120046
Unit / Price
  0.1 mg  /  $345 +1 FREE 8GB USB
Product Name

RB1, Monoclonal Antibody

Popular Item
Also Known As

Mouse monoclonal antibody Anti-Human RB1

Product Synonym Names
retinoblastoma 1 (including osteosarcoma) (RB1); RB; OSRC
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
4772
OMIM
109800
Clonality
Monoclonal
Isotype
IgG1
Clone Number
RB1AD56A
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Other Notes
Small volumes of anti-RB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RB1 antibody
Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression ('cure') occurs in some cases.[supplied by OMIM] [NCBI Entrez Gene Summary]
Applications Tested/Suitable for anti-RB1 antibody
Dot Blot (DB), ELISA (EIA), Immunocytochemistry (ICC)

Quality Control of anti-RB1 antibody
Western blot analysis of immunized recombinant protein, using anti-RB1 monoclonal antibody.
anti-RB1 antibody Quality Control image
Quality Control #2 of anti-RB1 antibody
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
anti-RB1 antibody Quality Control #2 image
Immunocytochemistry (ICC) of anti-RB1 antibody
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-RB1 mAb. [Lot No. RB1AD56A-3]
anti-RB1 antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for RB1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
106,159 Da
NCBI Official Full Name
Homo sapiens retinoblastoma 1 (RB1), mRNA
NCBI Official Synonym Full Names
retinoblastoma 1
NCBI Official Symbol
NCBI Official Synonym Symbols
RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
  [Similar Products]
NCBI Protein Information
retinoblastoma-associated protein; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; retinoblastoma suspectibility protein
UniProt Protein Name
Retinoblastoma-associated protein
UniProt Synonym Protein Names
p105-Rb; pRb; Rb; pp110
UniProt Gene Name
UniProt Entry Name
RB_HUMAN
NCBI Summary for RB1
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]
UniProt Comments for RB1
Rb: retinoblastoma tumor suppressor protein regulates cell proliferation by controlling progression through the G1-phase restriction point. Has three distinct binding domains and interacts with regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by Cdks inhibits Rb target binding, thus allowing cell cycle progression. Recruits and targets histone methyltransferase suv39h1 leading to epigenetic transcriptional repression.

Protein type: Tumor suppressor; Oncoprotein; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 13q14.2

Cellular Component: nucleoplasm; SWI/SNF complex; PML body; Rb-E2F complex; spindle; chromatin; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding; androgen receptor binding; ubiquitin protein ligase binding; transcription coactivator activity; phosphoprotein binding; kinase binding; transcription factor binding; transcription factor activity

Biological Process: sister chromatid biorientation; viral reproduction; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter, mitotic; regulation of mitotic cell cycle; neuron maturation; positive regulation of macrophage differentiation; cell cycle arrest; neurite development; negative regulation of epithelial cell proliferation; transcription, DNA-dependent; mitotic cell cycle checkpoint; negative regulation of transcription factor activity; enucleate erythrocyte differentiation; regulation of lipid kinase activity; myoblast differentiation; chromatin remodeling; G1/S-specific transcription in mitotic cell cycle; neuron apoptosis; maintenance of mitotic sister chromatid cohesion; gut development; cell division; negative regulation of smoothened signaling pathway; neuron morphogenesis during differentiation; androgen receptor signaling pathway; Ras protein signal transduction; positive regulation of mitotic metaphase/anaphase transition; striated muscle cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; cell cycle checkpoint; G1/S transition of mitotic cell cycle

Disease: Bladder Cancer; Osteogenic Sarcoma; Small Cell Cancer Of The Lung; Retinoblastoma
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000