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anti-RBPJ antibody :: Mouse anti-Human Recombining Binding Protein Suppressor of Hairless Monoclonal Antibody

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Catalog # MBS600710
Unit / Price
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  0.05 mg  /  $365 +1 FREE 8GB USB
anti-RBPJ antibody
Product Name

Recombining Binding Protein Suppressor of Hairless (RBPJ), Monoclonal Antibody

Full Product Name

Recombining Binding Protein Suppressor of Hairless (RBPSUH, IGKJRB, IGKJRB1, KBF2, MGC61669, RBP-J, RBPJK)

Product Synonym Names
Anti -Recombining Binding Protein Suppressor of Hairless (RBPSUH, IGKJRB, IGKJRB1, KBF2, MGC61669, RBP-J, RBPJK)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 4; NC_000004.11 (26321332..26433278). Location: 4p15.2
OMIM
147183
3D Structure
ModBase 3D Structure for Q06330
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C282
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes human Recombining Binding Protein Suppressor of Hairless.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Immunogen
Partial recombinant sequence from human Recombining Binding Protein Suppressor of Hairless (NM_015874).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Other Notes
Small volumes of anti-RBPJ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RBPJ antibody
RBPSUH (Recombining binding protein suppressor of hairless) is the DNA-binding component of the transcription complex regulated by Notch signaling. In the absence of Notch activation, RBPSUH suppresses target gene expression through interactions with a co-repressor complex containing histone deacetylase. Upon activation, the Notch intracellular domain (NICD) enters the nucleus and binds to RBPSUH to displace the co-repressor complex and replace with a transcription activation complex including Maml, histone acetylase p300, leading to the transcription of Notch target genes. In addition, RBPSUH is also the DNA-binding partner for Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2), a protein critical for latent viral transcription and immortalization of EBV-infected B cells.
Applications Tested/Suitable for anti-RBPJ antibody
Western Blot (WB), Gel Shift Assay (GS/EMSA)
Application Notes for anti-RBPJ antibody
Suitable for use in Western Blot and Dot Blot.
NCBI/Uniprot data below describe general gene information for RBPJ. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55,637 Da[Similar Products]
NCBI Official Full Name
recombining binding protein suppressor of hairless isoform 1
NCBI Official Synonym Full Names
recombination signal binding protein for immunoglobulin kappa J region
NCBI Official Symbol
NCBI Official Synonym Symbols
SUH; csl; CBF1; KBF2; RBP-J; RBPJK; IGKJRB; RBPSUH; IGKJRB1; MGC61669
  [Similar Products]
NCBI Protein Information
recombining binding protein suppressor of hairless; CBF-1; RBP-JK; RBP-J kappa; OTTHUMP00000123439; OTTHUMP00000123440; OTTHUMP00000123441; OTTHUMP00000217971; OTTHUMP00000217972; OTTHUMP00000217973; OTTHUMP00000218678; H-2K binding factor-2; suppressor of hairless homolog; renal carcinoma antigen NY-REN-30; j kappa-recombination signal-binding protein; immunoglobulin kappa J region recombination signal binding protein 1
UniProt Protein Name
Recombining binding protein suppressor of hairless
UniProt Synonym Protein Names
CBF-1; J kappa-recombination signal-binding protein; RBP-J kappa; RBP-J; RBP-JK; Renal carcinoma antigen NY-REN-30
UniProt Gene Name
UniProt Synonym Gene Names
IGKJRB; IGKJRB1; RBPJK; RBPSUH  [Similar Products]
UniProt Entry Name
SUH_HUMAN
NCBI Summary for RBPJ
The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013]
UniProt Comments for RBPJ
RBPJ: Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Interacts with activated NOTCH1, NOTCH2 or NOTCH3. Interacts with MINT/SHARP. This interaction may mediate the recruitment of large corepressor complexes containing proteins such as HDAC1, HDAC2, NCOR2, SAP30, FHL1/KYOT2 and CIR1. Interacts with EP300, MAML1 and PTF1A. Interacts with Epstein-Barr virus EBNA2, EBNA3, EBNA4 and EBNA6. Interacts with RITA/C12orf52, leading to nuclear export, prevent the interaction between RBPJ and NICD product and subsequent down-regulation of the Notch signaling pathway. Belongs to the Su(H) family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Transcription factor

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; recombinase activity; protein N-terminus binding; chromatin binding; transcription factor activity

Biological Process: somatic stem cell maintenance; myeloid dendritic cell differentiation; sebaceous gland development; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; negative regulation of cell proliferation; negative regulation of ossification; angiogenesis; regulation of timing of cell differentiation; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell proliferation; transcription initiation from RNA polymerase II promoter; epidermal cell fate specification; Notch signaling pathway; hair follicle maturation; humoral immune response; activation of Notch receptor target transcription factor; keratinocyte differentiation; inflammatory response to antigenic stimulus; pituitary gland development; B cell differentiation; defense response to bacterium; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; blood vessel remodeling; gene expression; negative regulation of transcription, DNA-dependent; auditory receptor cell fate commitment

Disease: Adams-oliver Syndrome 3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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