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anti-SERCA1 antibody :: Mouse anti-Human SERCA1 SERCA1 Monoclonal Antibody

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Catalog # MBS531944
Unit / Price
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  0.1 mL  /  $780 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

SERCA1, Monoclonal Antibody

Full Product Name

SERCA1 antibody

Product Synonym Names
Monoclonal SERCA1; Anti-SERCA1; SERCA1 ATPase; SER Calcium 2 ATPase (SERCA 1); Sarcoplasmic/Endoplasmic Reticulum Calcium 1 ATPase (SERCA 1); Anti SERCA1; SERCA1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9XU13
Clonality
Monoclonal
Isotype
IgG1
Clone Number
VE121G9
Host
Mouse
Species Reactivity
Human SERCA1
Form/Format
Ascites diluted in PBS with 0.05% NaN3.
Biological Significance
Antigen is purified rabbit skeletal muscle sarcoplasmic reticulum. This antibody recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen
SERCA1 antibody was raised in mouse using purified rabbit skeletal muscle sarcoplasmic reticulum as the immunogen.
Preparation and Storage
Aliquot and store at -20 degree C. Avoid repeated Freeze/Thaw cycles
Other Notes
Small volumes of anti-SERCA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SERCA1 antibody
Mouse monoclonal SERCA1 antibody
Applications Tested/Suitable for anti-SERCA1 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-SERCA1 antibody
IF: 1:500
WB: 1:2,500

Immunohistochemistry (IHC) of anti-SERCA1 antibody
Immunohistochemistry was performed on normal deparaffinized Human heart tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were probed at a dilution of 1:200 with SERCA1 antibody or without antibody (negative control) overnight at 4 deg C in a humidified chamber. Tissues were washed well with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
anti-SERCA1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for SERCA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
109,717 Da
NCBI Official Full Name
SCA-1, isoform b
NCBI Official Symbol
sca-1  [Similar Products]
NCBI Protein Information
SCA-1
UniProt Protein Name
Calcium-transporting ATPase
UniProt Gene Name
sca-1  [Similar Products]
UniProt Entry Name
Q9XU13_CAEEL
UniProt Comments for SERCA1
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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