View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List anti-SIRT1 antibody :: Rabbit anti-Human SIRT1 Monoclonal Antibody Catalog # MBS2544285 Unit / Price 0.06 mL / $260 +1 FREE 8GB USB 0.12 mL / $380 +1 FREE 8GB USB 0.2 mL / $560 +1 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name SIRT1, Monoclonal Antibody Full Product Name Rabbit anti Human SIRT1 Monoclonal Antibody Product Gene Name anti-SIRT1 antibody[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP OMIM AF083106 mRNA Clonality Monoclonal Isotype IgG Host Rabbit Species Reactivity Human Immunogen Recombinant Human SIRT1 protein Buffer 0.2 mum filtered solution in PBS with 5% trehalose Preparation and Storage This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. ISO Certification Manufactured in an ISO 9001:2015 Certified Laboratory. Other Notes Small volumes of anti-SIRT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-SIRT1 antibody SIRT1 belongs to the sirtuin family. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT1 is included in class I of the sirtuin family. It is a NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. It deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. SIRT1 also deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. It is involved in HES1- and HEY2-mediated transcriptional repression. SIRT1 inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. It may serve as a sensor of the cytosolic ratio of NAD (+)/NADH, which is essential in skeletal muscle cell differentiation. It also deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD (+)/NADP (+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Applications Tested/Suitable for anti-SIRT1 antibody ELISA (EIA) Application Notes for anti-SIRT1 antibody ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human SIRT1. The detection limit for Human SIRT1 is approximately 0.00975 ng/well. TOP NCBI/Uniprot data below describe general gene information for SIRT1. It may not necessarily be applicable to this product. NCBI GI # 15214730 NCBI GeneID 23411 UniProt Secondary Accession # Q2XNF6; Q5JVQ0; Q9GZR9; Q9Y6F0[Other Products] UniProt Related Accession # Q96EB6[Other Products] Molecular Weight 61,066 Da TOP NCBI Official Full Name SIRT1 protein NCBI Official Synonym Full Names sirtuin 1 NCBI Official Symbol SIRT1 [Similar Products] NCBI Official Synonym Symbols SIR2; SIR2L1; SIR2alpha [Similar Products] NCBI Protein Information NAD-dependent protein deacetylase sirtuin-1 UniProt Protein Name NAD-dependent protein deacetylase sirtuin-1 UniProt Synonym Protein Names Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2 Protein Family NAD-dependent protein deacetylase sirtuin UniProt Gene Name SIRT1 [Similar Products] UniProt Synonym Gene Names SIR2L1; hSIRT1; hSIR2; 75SirT1 [Similar Products] UniProt Entry Name SIR1_HUMAN TOP NCBI Summary for SIRT1 This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008] TOP UniProt Comments for SIRT1 SIRT1: an NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separate cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. Elevation of NAD(+)/NADP(+) ratio activates SIRT1. Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptional repression. Implicated in regulation of adipogenesis and fat mobilization in white adipocytes by repression of PPARG. Involved in liver and muscle metabolism. Is involved in autophagy, presumably by deacetylating ATG5, ATG7 and ATG8. Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation. Widely expressed. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Apoptosis; Deacetylase; EC 3.5.1.-; Nuclear receptor co-regulatorChromosomal Location of Human Ortholog: 10q21.3Cellular Component: chromatin silencing complex; cytoplasm; ESC/E(Z) complex; mitochondrion; nuclear chromatin; nuclear envelope; nuclear heterochromatin; nuclear inner membrane; nucleolus; nucleoplasm; nucleus; PML bodyMolecular Function: bHLH transcription factor binding; deacetylase activity; enzyme binding; histone binding; histone deacetylase activity; HLH domain binding; identical protein binding; mitogen-activated protein kinase binding; NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); nuclear hormone receptor binding; p53 binding; protein binding; protein C-terminus binding; protein deacetylase activity; transcription corepressor activity; transcription factor bindingBiological Process: angiogenesis; cell aging; cell glucose homeostasis; cellular response to starvation; cholesterol homeostasis; chromatin silencing; chromatin silencing at rDNA; circadian regulation of gene expression; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA repair; DNA replication; DNA synthesis during DNA repair; establishment and/or maintenance of chromatin architecture; establishment of chromatin silencing; fatty acid homeostasis; histone deacetylation; inhibition of NF-kappaB transcription factor; leptin-mediated signaling pathway; macrophage differentiation; maintenance of chromatin silencing; methylation-dependent chromatin silencing; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of fat cell differentiation; negative regulation of helicase activity; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of phosphorylation; negative regulation of prostaglandin biosynthetic process; negative regulation of protein kinase B signaling cascade; negative regulation of TOR signaling pathway; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; peptidyl-lysine acetylation; positive regulation of adaptive immune response; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of cell proliferation; positive regulation of chromatin silencing; positive regulation of DNA repair; positive regulation of endothelial cell proliferation; positive regulation of histone H3-K9 methylation; positive regulation of insulin receptor signaling pathway; positive regulation of macroautophagy; positive regulation of MHC class II biosynthetic process; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid ADP-ribosylation; protein amino acid deacetylation; protein destabilization; protein ubiquitination; pyrimidine dimer repair via nucleotide-excision repair; regulation of cell proliferation; regulation of endodeoxyribonuclease activity; regulation of mitotic cell cycle; regulation of protein import into nucleus, translocation; response to DNA damage stimulus; response to hydrogen peroxide; response to insulin stimulus; response to oxidative stress; single strand break repair; triacylglycerol mobilization; white fat cell differentiation Research Articles on SIRT1 1. High expression of Sirt1 implies a poor prognosis of gastric cancer patients in a relatively short period (3 years), but not in a long time (>/=5 years). The expression of Sirt1 is also linked with patients' age, T stage, N stage and tumor differentiation. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. 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TOP Products associated with anti-SIRT1 antibody Reference Product PubMed Publications PPARGC1A antibody >253 publications with SIRT1 and PPARGC1A FOXO1 antibody >224 publications with SIRT1 and FOXO1 FOXO3 antibody >108 publications with SIRT1 and FOXO3 TP53 antibody >82 publications with SIRT1 and TP53 KIAA1967 antibody >26 publications with SIRT1 and KIAA1967 EP300 antibody >22 publications with SIRT1 and EP300 XRCC6 antibody >17 publications with SIRT1 and XRCC6 FOXO4 antibody >15 publications with SIRT1 and FOXO4 RPS19BP1 antibody >10 publications with SIRT1 and RPS19BP1 KAT2B antibody >2 publications with SIRT1 and KAT2B Pathways associated with anti-SIRT1 antibody Products by Pathway Pathway Diagram AMPK Signaling Pathway antibodies AMPK Signaling Pathway Diagram AMPK Signaling Pathway antibodies AMPK Signaling Pathway Diagram Amphetamine Addiction Pathway antibodies Amphetamine Addiction Pathway Diagram Amphetamine Addiction Pathway antibodies Amphetamine Addiction Pathway Diagram Androgen Receptor Signaling Pathway antibodies Androgen Receptor Signaling Pathway Diagram Cellular Response To Heat Stress Pathway antibodies Cellular Response To Heat Stress Pathway Diagram Cellular Responses To Stress Pathway antibodies Cellular Responses To Stress Pathway Diagram Circadian Clock Pathway antibodies Circadian Clock Pathway Diagram Circadian Rythm Related Genes Pathway antibodies Circadian Rythm Related Genes Pathway Diagram E2F Transcription Factor Network Pathway antibodies E2F Transcription Factor Network Pathway Diagram Diseases associated with anti-SIRT1 antibody Disease Name Pubmed Publications Inflammation Antibodies >599 publications with SIRT1 and Inflammation Obesity Antibodies >416 publications with SIRT1 and Obesity Insulin Resistance Antibodies >319 publications with SIRT1 and Insulin Resistance Diabetes Mellitus Antibodies >286 publications with SIRT1 and Diabetes Mellitus Neurodegenerative Diseases Antibodies >257 publications with SIRT1 and Neurodegenerative Diseases Fatty Liver Antibodies >239 publications with SIRT1 and Fatty Liver Necrosis Antibodies >206 publications with SIRT1 and Necrosis Heart Diseases Antibodies >199 publications with SIRT1 and Heart Diseases Atherosclerosis Antibodies >138 publications with SIRT1 and Atherosclerosis Diabetes Mellitus, Type 2 Antibodies >135 publications with SIRT1 and Diabetes Mellitus, Type 2 Organs/Tissues associated with anti-SIRT1 antibody Organ/Tissue Name Pubmed Publications Liver Antibodies >644 publications with SIRT1 and Liver Muscle Antibodies >620 publications with SIRT1 and Muscle Brain Antibodies >503 publications with SIRT1 and Brain Vascular Antibodies >404 publications with SIRT1 and Vascular Kidney Antibodies >207 publications with SIRT1 and Kidney Lung Antibodies >202 publications with SIRT1 and Lung Bone Antibodies >164 publications with SIRT1 and Bone Skin Antibodies >98 publications with SIRT1 and Skin Eye Antibodies >83 publications with SIRT1 and Eye Pancreas Antibodies >82 publications with SIRT1 and Pancreas TOP