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anti-Tshr antibody :: Mouse anti-Human Thyrotrophin Receptor Monoclonal Antibody

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Catalog # MBS603375
Unit / Price
  0.2 mg  /  $890 +1 FREE 8GB USB
anti-Tshr antibody
Product Name

Thyrotrophin Receptor (Tshr), Monoclonal Antibody

Popular Item
Also Known As

Thyrotrophin Receptor

Product Synonym Names
Anti -Thyrotrophin Receptor
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 6; NC_005105.2 (115024999..115162531). Location: 6q31
3D Structure
ModBase 3D Structure for P21463
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3H3042
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes the human thyrotrophin receptor, binding to an extracellular domain.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Immunogen
External domain of human thyrotrophin receptor linked to GST.
Recommended Negative Controls
Mouse IgG2a Negative Control
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the NS1/Ag4.1 myeloma cell line.
Preparation and Storage
-20 degree C
Other Notes
Small volumes of anti-Tshr antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forTshrpurchase
GPCRs,- a multitude of extracellular signaling transducers. Teamed with their intracellular partners - G-proteins- they are integral in homeostasis and imbalances can create conditions resulting in clinical human autoimmune pathologies such as Graves disease and Hashimoto thyroiditis. These autoimmune thyroid disease (s) results from generation of antibodies anti thyroid stimulating hormone (thyrotropin TSH) which can also bind and stimulate the thyrotropin receptor (TSHR) and TSH receptor. Translational and therapeutic research, targeted to thyrotropin receptor and not related family gene members with conserved domain structures, requires highly selective custom antibody production to generate our receptor antibody. MyBiosource has a mouse mAb ideal for research applications of cell lysates with broad AMP secondary antibodies detection. This product recognizes an extracellular domain distinct from other protein family antibodies based on Pubmed alignment tool BLAST database bioinformatics. Compare our value to other TSH receptor or thyroid antibodies and order today at 866 697 8378.
Applications Tested/Suitable for anti-Tshr antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-Tshr antibody
Flow Cytometry: 1:100-1:1000, Western Blotting: 1:1000, Immunohistochemistry: Frozen, Paraffin, Immunoprecipitation, Optimal working dilutions to be determined by researcher.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
NCBI/Uniprot data below describe general gene information for Tshr. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
86,475 Da[Similar Products]
NCBI Official Full Name
thyrotropin receptor
NCBI Official Synonym Full Names
thyroid stimulating hormone receptor
NCBI Official Symbol
NCBI Official Synonym Symbols
TSHRA
  [Similar Products]
NCBI Protein Information
thyrotropin receptor; TSH-R; thyroid-stimulating hormone receptor
UniProt Protein Name
Thyrotropin receptor
UniProt Synonym Protein Names
Thyroid-stimulating hormone receptor
Protein Family
UniProt Gene Name
UniProt Entry Name
TSHR_RAT
NCBI Summary for Tshr
acts as a G-protein coupled receptor for thyroid-stimulating hormone; induces increased cAMP levels [RGD]
UniProt Comments for Tshr
TSHR: Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5). Defects in TSHR are found in patients affected by hyperthyroidism with different etiologies. Somatic, constitutively activating TSHR mutations and/or constitutively activating G(s)alpha mutations have been identified in toxic thyroid nodules (TTNs) that are the predominant cause of hyperthyroidism in iodine deficient areas. These mutations lead to TSH independent activation of the cAMP cascade resulting in thyroid growth and hormone production. TSHR mutations are found in autonomously functioning thyroid nodules (AFTN), toxic multinodular goiter (TMNG) and hyperfunctioning thyroid adenomas (HTA). TMNG encompasses a spectrum of different clinical entities, ranging from a single hyperfunctioning nodule within an enlarged thyroid, to multiple hyperfunctioning areas scattered throughout the gland. HTA are discrete encapsulated neoplasms characterized by TSH- independent autonomous growth, hypersecretion of thyroid hormones, and TSH suppression. Defects in TSHR are also a cause of thyroid neoplasms (papillary and follicular cancers). Autoantibodies against TSHR are directly responsible for the pathogenesis and hyperthyroidism of Graves disease. Antibody interaction with TSHR results in an uncontrolled receptor stimulation. Defects in TSHR are the cause of congenital hypothyroidism non-goitrous type 1 (CHNG1); also known as congenital hypothyroidism due to TSH resistance. CHNG1 is a non-autoimmune condition characterized by resistance to thyroid- stimulating hormone (TSH) leading to increased levels of plasma TSH and low levels of thyroid hormone. CHNG1 presents variable severity depending on the completeness of the defect. Most patients are euthyroid and asymptomatic, with a normal sized thyroid gland. Only a subset of patients develop hypothyroidism and present a hypoplastic thyroid gland. Defects in TSHR are the cause of familial gestational hyperthyroidism (HTFG). HTFG is a condition characterized by abnormally high levels of serum thyroid hormones occurring during early pregnancy. Defects in TSHR are the cause of hyperthyroidism non- autoimmune (HTNA). It is a condition characterized by abnormally high levels of serum thyroid hormones, thyroid hyperplasia, goiter and lack of anti-thyroid antibodies. Typical features of Graves disease such as exophthalmia, myxedema, antibodies anti-TSH receptor and lymphocytic infiltration of the thyroid gland are absent. Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Cellular Component: integral to membrane; plasma membrane; receptor complex; lipid raft

Molecular Function: thyroid-stimulating hormone receptor activity

Biological Process: regulation of locomotion; inner ear receptor stereocilium organization and biogenesis; adult locomotory behavior; inner ear receptor cell development; B cell differentiation; positive regulation of cell proliferation; positive regulation of multicellular organism growth; G-protein signaling, adenylate cyclase activating pathway
Research Articles on Tshr
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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