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anti-TRIM28 antibody :: Mouse anti-Human TRIM28 Monoclonal Antibody

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Catalog # MBS120011
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Product Name

TRIM28, Monoclonal Antibody

Popular Item
Full Product Name

Mouse monoclonal antibody Anti-Human TRIM28

Product Synonym Names
tripartite motif-containing 28 (TRIM28); KAP1; TF1B; RNF96; TIF1B
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
601742
Clonality
Monoclonal
Isotype
IgG1
Clone Number
TRI5I093
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Other Notes
Small volumes of anti-TRIM28 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TRIM28 antibody
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [NCBI Entrez Gene Summary]
Applications Tested/Suitable for anti-TRIM28 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB)

Quality Control of anti-TRIM28 antibody
Left: Western blot analysis of immunized recombinant protein, using anti-TRIM28 monoclonal antibody.
anti-TRIM28 antibody Quality Control image
Western Blot (WB) of anti-TRIM28 antibody
Detection of TRIM28 by Western blot.

Samples: Whole cell lysate from human HEK293 (H, 25 ug) and mouse NIH3T3 (M, 25 ug) cells. [Lot No. TRI5I093-2]

Predicted molecular weight: 88 kDa
anti-TRIM28 antibody Western Blot (WB) (WB) image
Immunocytochemistry (ICC) of anti-TRIM28 antibody
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TRIM28 mAb. [Lot No. TRI5I093-2]
anti-TRIM28 antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for TRIM28. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
79,474 Da
NCBI Official Full Name
Homo sapiens tripartite motif containing 28 (TRIM28), mRNA
NCBI Official Synonym Full Names
tripartite motif containing 28
NCBI Official Symbol
TRIM28  [Similar Products]
NCBI Official Synonym Symbols
KAP1; TF1B; RNF96; TIF1B; PPP1R157
  [Similar Products]
NCBI Protein Information
transcription intermediary factor 1-beta; E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; RING finger protein 96; TIF1-beta; nuclear corepressor KAP-1; protein phosphatase 1, regulatory subunit 157; transcriptional intermediary factor 1-beta; tripartite motif-containing 28; tripartite motif-containing protein 28
UniProt Protein Name
Transcription intermediary factor 1-beta
UniProt Synonym Protein Names
E3 SUMO-protein ligase TRIM28 (EC:6.3.2.-); KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; RING finger protein 96; Tripartite motif-containing protein 28
UniProt Gene Name
TRIM28  [Similar Products]
UniProt Entry Name
TIF1B_HUMAN
NCBI Summary for TRIM28
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
UniProt Comments for TRIM28
TIF1B: an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Protein type: Kinase, protein; Transcription, coactivator/corepressor; Ubiquitin ligase; Protein kinase, atypical; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Nuclear receptor co-regulator; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: nucleoplasm; nuclear heterochromatin; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; transcription corepressor activity; transcription factor activity; ligase activity; protein kinase activity

Biological Process: transcription initiation from RNA polymerase II promoter; convergent extension involved in axis elongation; positive regulation of DNA repair; protein amino acid autophosphorylation; positive regulation of transcription, DNA-dependent; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein oligomerization; protein sumoylation; positive regulation of transcription factor import into nucleus; epithelial to mesenchymal transition; innate immune response; gene expression; negative regulation of transcription, DNA-dependent; DNA methylation during embryonic development; embryo implantation
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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