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anti-TWIST antibody :: Mouse anti-Human TWIST Monoclonal Antibody

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Catalog # MBS120163
Unit / Price
  0.1 mg  /  $345 +1 FREE 8GB USB
Product Name

TWIST, Monoclonal Antibody

Popular Item
Also Known As

Mouse monoclonal antibody Anti-Human TWIST

Product Synonym Names
Homo sapiens twist homolog 1 (acrocephalosyndactyly 3; Saethre-Chotzen syndrome) (Drosophila) (TWIST1); SCS;ACS3; BPES2; BPES3;TWIST1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1669
OMIM
101400
Clonality
Monoclonal
Isotype
IgG1
Clone Number
Twist2C1a
Host
Mouse
Species Reactivity
Human
Concentration
100 ug/ml (1.0 ml) (lot specific)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
Other Notes
Small volumes of anti-TWIST antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TWIST antibody
Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [NCBI Entrez Gene Summary]
Applications Tested/Suitable for anti-TWIST antibody
Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)

Quality Control of anti-TWIST antibody
Western blot analysis of immunized recombinant protein, using anti-TWIST monoclonal antibody.
anti-TWIST antibody Quality Control image
Quality Control #2 of anti-TWIST antibody
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
anti-TWIST antibody Quality Control #2 image
Western Blot (WB) of anti-TWIST antibody
Detection of TWIST by Western blot.

Samples: Whole cell lysate from human A2058 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. Twist2C1a-1]

Predicted molecular weight: 20 kDa
anti-TWIST antibody Western Blot (WB) (WB) image
Flow Cytometry (FC/FACS) of anti-TWIST antibody
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-TWIST mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. Twist2C1a-1]
anti-TWIST antibody Flow Cytometry (FC/FACS) (FC/FACS) image
Immunocytochemistry (ICC) of anti-TWIST antibody
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-TWIST mAb. [Lot No. Twist2C1a-1]
anti-TWIST antibody Immunocytochemistry (ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for TWIST. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,954 Da
NCBI Official Full Name
Homo sapiens twist family bHLH transcription factor 1 (TWIST1), mRNA
NCBI Official Synonym Full Names
twist family bHLH transcription factor 1
NCBI Official Symbol
TWIST1  [Similar Products]
NCBI Official Synonym Symbols
CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
  [Similar Products]
NCBI Protein Information
twist-related protein 1; B-HLH DNA binding protein; H-twist; TWIST homolog of drosophila; class A basic helix-loop-helix protein 38; twist basic helix-loop-helix transcription factor 1; twist homolog 1
UniProt Protein Name
Twist-related protein 1
UniProt Synonym Protein Names
Class A basic helix-loop-helix protein 38; bHLHa38; H-twist
Protein Family
UniProt Gene Name
TWIST1  [Similar Products]
UniProt Entry Name
TWST1_HUMAN
NCBI Summary for TWIST
Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq, Jul 2008]
UniProt Comments for TWIST
TWIST1: Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed. Subset of mesodermal cells.

Protein type: Transcription regulation; Cell development/differentiation; Inhibitor; DNA-binding

Chromosomal Location of Human Ortholog: 7p21.2

Cellular Component: nucleus

Molecular Function: protein domain specific binding; protein binding; protein homodimerization activity; protein heterodimerization activity; bHLH transcription factor binding; transcription factor binding

Biological Process: embryonic forelimb morphogenesis; transcription from RNA polymerase II promoter; negative regulation of skeletal muscle development; muscle development; neuron migration; rhythmic process; negative regulation of transcription from RNA polymerase II promoter; palate development; embryonic hindlimb morphogenesis; negative regulation of histone phosphorylation; negative regulation of histone acetylation; odontogenesis; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of osteoblast differentiation; ossification; in utero embryonic development; negative regulation of transcription factor activity; outer ear morphogenesis; negative regulation of tumor necrosis factor production; embryonic cranial skeleton morphogenesis; positive regulation of tumor necrosis factor production; positive regulation of fatty acid beta-oxidation; osteoblast differentiation; positive regulation of angiogenesis; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; regulation of bone mineralization; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of phosphoinositide 3-kinase cascade

Disease: Robinow-sorauf Syndrome; Craniosynostosis 1; Saethre-chotzen Syndrome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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