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anti-TYR antibody :: Mouse Tyrosinase Monoclonal Antibody

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Catalog # MBS438285
Unit / Price
Scan QR to view Datasheet
  0.02 mg (With BSA & Azide at 0.2mg/ml)  /  $190 +1 FREE 8GB USB
  7 mL (Prediluted)  /  $270 +1 FREE 8GB USB
  0.1 mg (With BSA & Azide at 0.2mg/ml)  /  $300 +1 FREE 8GB USB
  0.1 mg (Without BSA & Azide at 1mg/ml)  /  $310 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

Tyrosinase, Monoclonal Antibody

Popular Item
Full Product Name

Tyrosinase (Melanoma Marker) Mouse Monoclonal Antibody

Product Synonym Names
ATN, CMM8, LB24-AB, Monophenol monooxygenase, OCA1, OCA1A, Oculocutaneous albinism IA, SHEP3, SK29-AB, Tumor rejection antigen AB, TYR
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
103470
3D Structure
ModBase 3D Structure for P14679
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
SPM360
Host
Mouse
Species Reactivity
Human. Others not known.
Specificity
Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Cellular Localization
Cytoplasmic
Immunogen
Recombinant tyrosinase protein
Hu-Chromosome Location
11q14.3
Positive Control
SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.
Preparation and Storage
Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-TYR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TYR antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-TYR antibody
Flow Cytometry (0.5-1ug/million cells )
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5,for 10-20 ming followed by cooling at RT for 20 min).

Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-TYR antibody
Formalin-fixed, paraffin-embedded human Melanoma stained with Tyrosinase Monoclonal Antibody (SPM360).
anti-TYR antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for TYR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
70-80kDa
NCBI Official Full Name
tyrosinase
NCBI Official Synonym Full Names
tyrosinase
NCBI Official Symbol
NCBI Official Synonym Symbols
ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3
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NCBI Protein Information
tyrosinase
UniProt Protein Name
Tyrosinase
UniProt Synonym Protein Names
LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB
Protein Family
UniProt Gene Name
UniProt Entry Name
TYRO_HUMAN
NCBI Summary for TYR
The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008]
UniProt Comments for TYR
TYR: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. Increased expression after UVB irradiation. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.18.1; Oxidoreductase; Amino Acid Metabolism - tyrosine; Membrane protein, integral; Cofactor and Vitamin Metabolism - riboflavin

Chromosomal Location of Human Ortholog: 11q14.3

Cellular Component: cytoplasm; Golgi-associated vesicle; lysosome; melanosome; melanosome membrane; perinuclear region of cytoplasm

Molecular Function: copper ion binding; monooxygenase activity; monophenol monooxygenase activity; protein binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: eye pigment biosynthetic process; melanin biosynthetic process; melanin biosynthetic process from tyrosine; visual perception

Disease: Albinism, Ocular, With Sensorineural Deafness; Albinism, Oculocutaneous, Type Ia; Albinism, Oculocutaneous, Type Ib; Skin/hair/eye Pigmentation, Variation In, 3
Product References and Citations for anti-TYR antibody
Chen Y-T, et. al. Proc. Natl. Acad. Sci. USA, 1995, 92:8125-8129

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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