• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-UBE2S antibody :: Mouse anti-Human UBE2S Monoclonal Antibody

Scan QR to view Datasheet
Catalog # MBS200167
Unit / Price
  0.05 mL  /  $240 +1 FREE 8GB USB
  0.1 mL  /  $315 +1 FREE 8GB USB
Product Name

UBE2S, Monoclonal Antibody

Popular Item
Also Known As

UBE2S antibody

Product Synonym Names
ubiquitin-conjugating enzyme E2S; E2-EPF; E2EPF; EPF5; ubiquitin-conjugating enzyme E2S AA409170; MGC101982; uBE2S; ubiquitin carrier protein S; ubiquitin protein ligase S; ubiquitin conjugating enzyme E2 24 kD; ubiquitin conjugating enzyme E2S
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
222
OMIM
M91670 mRNA
3D Structure
ModBase 3D Structure for Q16763
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT2C12
Host
Mouse
Species Reactivity
Human
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Antigen Species
Human
Immunogen
Recombinant human UBE2S (1-222aa) purified from E. coli
Clone Note
Anti-human UBE2S mAb, clone AT2C12, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human UBE2S protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-UBE2S antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-UBE2S antibody
UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins.
Product Categories/Family for anti-UBE2S antibody
Applications Tested/Suitable for anti-UBE2S antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-UBE2S antibody
WB/IF: 1:250 ~ 500.
Recommended starting dilution is 1:250

Western Blot (WB) of anti-UBE2S antibody
The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2S antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: UBE2S transfected 293T cell lysate
anti-UBE2S antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-UBE2S antibody
Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human UBE2S antibody (1:500) with Alexa 488 (Green).
anti-UBE2S antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for UBE2S. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
23,845 Da
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 S
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 S
NCBI Official Symbol
UBE2S  [Similar Products]
NCBI Official Synonym Symbols
EPF5; E2EPF; E2-EPF
  [Similar Products]
NCBI Protein Information
ubiquitin-conjugating enzyme E2 S
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 S
UniProt Synonym Protein Names
E2 ubiquitin-conjugating enzyme S; E2-EPF; Ubiquitin carrier protein S; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; Ubiquitin-protein ligase S
UniProt Gene Name
UBE2S  [Similar Products]
UniProt Synonym Gene Names
E2EPF  [Similar Products]
NCBI Summary for UBE2S
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]
UniProt Comments for UBE2S
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:22496338). Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (PubMed:19820702, PubMed:19822757, PubMed:27259151). Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A (PubMed:16819549). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination (PubMed:20061386, PubMed:20622874).
Product References and Citations for anti-UBE2S antibody
Ye Y, et al. (2009) Nat Rev Mol Cell Biol, 10 (11):755-64.; Windheim M, et al. (2008) Biochem J, 409 (3):723-9.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000