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anti-UBE2I antibody :: Mouse anti-Human Ubiquitin Conjugating Enzyme E2L6 Monoclonal Antibody

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Catalog # MBS607995
Unit / Price
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  0.1 mL  /  $490 +1 FREE 8GB USB
anti-UBE2I antibody
Product Name

Ubiquitin Conjugating Enzyme E2L6 (UBE2I), Monoclonal Antibody

Full Product Name

Ubiquitin Conjugating Enzyme E2L6 (UBE2L6, UbcH8)

Product Synonym Names
Anti -Ubiquitin Conjugating Enzyme E2L6 (UBE2L6, UbcH8)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 16; NC_000016.9 (1359180..1375390). Location: 16p13.3
OMIM
601661
3D Structure
ModBase 3D Structure for P63279
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
10C318
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes human UBE2L6 (1-152aa)
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied in liquid in PBS, pH 7.4, 0.1% sodium azide.
Immunogen
Recombinant human UBE2L6 (1-152aa) purified from E. coli
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-UBE2I antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-UBE2I antibody
Ubiquitin-conjugating enzyme E2L6 (UBE2L6), also known as UbcH8, is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination of a protein substrate requires the concerted action of 3 classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. The E2 ubiquitin-conjugating enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene.
Product Categories/Family for anti-UBE2I antibody
Applications Tested/Suitable for anti-UBE2I antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-UBE2I antibody
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:1000-1:3000
immunohistochemistry: 1:50-1:100
NCBI/Uniprot data below describe general gene information for UBE2I. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,007 Da[Similar Products]
NCBI Official Full Name
ubiquitin conjugating enzyme
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2I
NCBI Official Symbol
UBE2I  [Similar Products]
NCBI Official Synonym Symbols
P18; UBC9; C358B7.1
  [Similar Products]
NCBI Protein Information
SUMO-conjugating enzyme UBC9; OTTHUMP00000045512; OTTHUMP00000158627; OTTHUMP00000158628; OTTHUMP00000158629; OTTHUMP00000198126; OTTHUMP00000198127; OTTHUMP00000198131; SUMO-protein ligase; SUMO-1-protein ligase; ubiquitin-protein ligase I; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin-protein ligase E2I; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
UniProt Protein Name
SUMO-conjugating enzyme UBC9
UniProt Synonym Protein Names
SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18
UniProt Gene Name
UBE2I  [Similar Products]
UniProt Synonym Gene Names
UBC9; UBCE9  [Similar Products]
UniProt Entry Name
UBC9_HUMAN
NCBI Summary for UBE2I
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
UniProt Comments for UBE2I
UBC9: Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Interacts with HIPK1, HIPK2, PPM1J, RASD2 and TCF3 Interacts with NR2C1; the interaction promotes its sumoylation. Forms a tight complex with RANGAP1 and RANBP2. Interacts with SIAH1 and PARP. Interacts with various transcription factors such as TFAP2A, TFAP2B, TFAP2C, AR, ETS1 and SOX4. Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B. Interacts with DNMT1. Interacts with FOXL2. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Interacts with human herpesvirus 6 IE2. Interacts with human adenovirus early E1A protein; this interaction interferes with polysumoylation (Probable). Interacts with DNM1l (via its GTPase and B domains); the interaction promotes sumoylation of DNM1L, mainly in its B domain. Interacts with PML-RARA oncoprotein (via the coiled-colied domain); the interaction is required for sumoylation of the PML- RARA oncoprotein. Interacts with IPO13. Interacts with NFATC2IP; this inhibits formation of poly-SUMO chains. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: SUMO conjugating system; EC 6.3.2.19; Ligase; Ubiquitin ligase; Nuclear receptor co-regulator; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; PML body; dendrite; cytoplasm; fibrillar center; synapse; synaptonemal complex; nucleus

Molecular Function: protein C-terminus binding; protein binding; enzyme binding; ubiquitin protein ligase binding; bHLH transcription factor binding; transcription factor binding; HLH domain binding; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; mitosis; viral reproduction; positive regulation of steroid hormone receptor signaling pathway; protein ubiquitination; protein modification process; negative regulation of transcription from RNA polymerase II promoter; post-translational protein modification; chromosome segregation; cellular protein metabolic process; protein sumoylation; cell division; positive regulation of transcription factor activity; regulation of receptor activity; negative regulation of transcription, DNA-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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