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anti-VCP antibody :: Mouse anti-Human VCP Monoclonal Antibody

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Catalog # MBS245142
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

VCP, Monoclonal Antibody

Also Known As

Mouse Monoclonal [clone 4G9] (IgG1) to Human VCP

Product Synonym Names
Anti-VCP Antibody (clone 4G9) IHC-plus; VCP; 15S Mg(2+)-ATPase p97 subunit; ALS14; TER ATPase; TERA; Valosin containing protein; Yeast Cdc48p homolog; Valosin-containing protein; IBMPFD; p97; Human VCP
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
806
OMIM
gene 613954
3D Structure
ModBase 3D Structure for P55072
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4G9
Host
Mouse
Species Reactivity
Human
Specificity
Recombinant human valosin containing protein
Purity/Purification
Affinity Purified
Form/Format
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml. Sourced in TCS.
Concentration
0.5 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Recombinant human VCP.
Immunogen Type
Recombinant protein
Immunogen
VCP antibody was raised against recombinant human VCP.
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
Other Notes
Small volumes of anti-VCP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-VCP antibody
Valsolin Containing Protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex.
Applications Tested/Suitable for anti-VCP antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-VCP antibody
IF, IHC-P (5 ug/ml), IP, WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-VCP antibody
Anti-VCP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-VCP antibody Immunohistochemistry (IHC) (IHC) image
Immunocytochemistry (ICC) of anti-VCP antibody
Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against p97/VCP (clone 4G9), followed by Cy3 conjugated goat anti-mouse IgG and DAPI
anti-VCP antibody Immunocytochemistry (ICC) (ICC) image
Western Blot (WB) of anti-VCP antibody
Western blot of extracts from HeLa cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9
anti-VCP antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for VCP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
89,322 Da
NCBI Official Full Name
transitional endoplasmic reticulum ATPase
NCBI Official Synonym Full Names
valosin containing protein
NCBI Official Symbol
NCBI Official Synonym Symbols
p97; TERA; ALS14; IBMPFD; HEL-220; IBMPFD1; HEL-S-70
  [Similar Products]
NCBI Protein Information
transitional endoplasmic reticulum ATPase; TER ATPase; yeast Cdc48p homolog; valosin-containing protein; 15S Mg(2+)-ATPase p97 subunit; epididymis luminal protein 220; epididymis secretory protein Li 70
UniProt Protein Name
Transitional endoplasmic reticulum ATPase
UniProt Synonym Protein Names
15S Mg(2+)-ATPase p97 subunit; Valosin-containing protein; VCP
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
TER ATPase; VCP  [Similar Products]
UniProt Entry Name
TERA_HUMAN
NCBI Summary for VCP
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq, Jul 2008]
UniProt Comments for VCP
VCP: valosin-containing protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope and of the transitional endoplasmic reticulum (tER). Regulates NFKappaB pathway, which is important for metastasis of osteosarcoma. Tyrosine phosphorylation regulates its cell cycle-dependent nuclear localization.

Protein type: DNA repair, damage; Endoplasmic reticulum; Chaperone; EC 3.6.4.6; Hydrolase

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: proteasome complex; nucleoplasm; endoplasmic reticulum membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; lipid particle; cytosol; nucleus

Molecular Function: protein domain specific binding; identical protein binding; protein binding; ATPase activity; protein complex binding; polyubiquitin binding; ADP binding; protein phosphatase binding; lipid binding; ATP binding; receptor binding

Biological Process: caspase activation; proteasomal ubiquitin-dependent protein catabolic process; ER-associated protein catabolic process; ER to Golgi vesicle-mediated transport; unfolded protein response; protein ubiquitination; DNA repair; regulation of apoptosis; retrograde protein transport, ER to cytosol; establishment of protein localization; bypass DNA synthesis; positive regulation of protein complex assembly; double-strand break repair; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; protein amino acid N-linked glycosylation via asparagine; response to DNA damage stimulus; protein homooligomerization

Disease: Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 1; Amyotrophic Lateral Sclerosis 14, With Or Without Frontotemporal Dementia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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